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07-496-I Anti-Myeloperoxidase Antibody

Anti-Myeloperoxidase Antibody, Cat. No. 07-496-I, is a highly specific rabbit polyclonal antibody that targets Myeloperoxidase and has been tested in Flow Cytometry and Western Blotting.

Anti-Myeloperoxidase Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: MPO UniProt Number: P05164
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07-496-I
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-496-I
Replaces07-496
DescriptionAnti-Myeloperoxidase Antibody
Alternate Names
  • Myeloperoxidase
  • myeloperoxidase heavy chain
Background InformationMyeloperoxidase (EC 1.11.2.2; UniProt P05164) is encoded by the MPO gene (Gene ID 4353) in human. Myeloperoxidase/MPO is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a 745 a.a. single chain precursor with a signal peptide sequence (a.a. 1-48), myeloperoxidase is subsequently cleaved into a light chain (a.a.165-278) and a heavy chain (a.a. 279-745). The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. MPO catalyzes hydrogen peroxide-dependent oxidations and the formation of hypochlorous acid that is central to the microbicidal activity of netrophils.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Myeloperoxidase Antibody, Cat. No. 07-496-I, is a highly specific rabbit polyclonal antibody that targets Myeloperoxidase and has been tested in Flow Cytometry and Western Blotting.
Key Applications
  • Flow Cytometry
  • Western Blotting
Application NotesFlow Cytometry Analysis: 0.1 µg from a representative lot detected myeloperoxidase immunoreactivity in 4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized HL-60 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence within human myeloperoxidase heavy chain region.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThe immunogen sequence is present in the pro-fom and the mature forms of myeloperoxidase (without a.a. 1-48, a.a. 1-154, or a.a. 1-164), as well as the cleaved heavy chain (a.a. 279-745), but not light chain (a.a. 165-278).
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • MPO
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~92/60 kDa (glycosylated pro-form/heavy chain) observed. 83.87/73.85/87.25 kDa (isoform H17/H14/H7 pro-form), 78.94/82.32 kDa (isoform H17/H7 with a.a. 1-48 propeptide removed), 66.45/66.45/69.83 kDa (isoform H17/H14/H7 with a.a. 1-154 removed), 65.48/65.48/68.86 kDa (isoform H17/H7 isoform H17/H14/H7 with a.a. 1-164 removed), 52.51 kDa (heavy chain; a.a. 279-745) calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected myeloperoxidase heavy chain in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-496-I 04054839090271

Documentation

Anti-Myeloperoxidase Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Myeloperoxidase Antibody Certificates of Analysis

TitleLot Number
Anti-Myeloperoxidase - 3261269 3261269
Anti-Myeloperoxidase - 3318596 3318596
Anti-Myeloperoxidase - 3475639 3475639
Anti-Myeloperoxidase - 3794314 3794314
Anti-Myeloperoxidase - 3874288 3874288
Anti-Myeloperoxidase - 4059294 4059294
Anti-Myeloperoxidase - 4123393 4123393
Anti-Myeloperoxidase - 4185132 4185132
Anti-Myeloperoxidase -Q2774356 Q2774356
Anti-Myeloperoxidase Polyclonal Antibody 3162407
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