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OP13 Anti-N-Myc Mouse mAb (NCM II 100)

This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.

Anti-N-Myc Mouse mAb (NCM II 100) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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OP13
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
OP13-100UG Plastic ampoule 100 μg
Description
OverviewRecognizes the N-myc protein and its cleavage product in IMR5 cells.
Catalogue NumberOP13
Brand Family Calbiochem®
References
ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
Slamon, D.J., et al. 1986. Science 232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
Brodeur, G.M., et al. 1984. Science 224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
Kohl, N.E., et al. 1983. Cell 35, 603.
Schwab, M., et al. 1983. Nature 305, 245.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
Positive controlIMR5 cells
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Immunofluorescence
Immunoprecipitation
Application NotesFrozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml, see application references)
Immunofluorescence (1-5 µg/ml, see application references)
Immunoprecipitation (1-2 µg/sample, see application references)
Application CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant, human, N-myc fusion protein, expressed in E. coli
ImmunogenHuman
CloneNCM II 100
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
OP13-100UG 04055977224795

Documentation

Anti-N-Myc Mouse mAb (NCM II 100) SDS

Title

Safety Data Sheet (SDS) 

Anti-N-Myc Mouse mAb (NCM II 100) Certificates of Analysis

TitleLot Number
OP13

References

Reference overview
Brondyk, W.H. 1991. Oncogene 6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
Slamon, D.J., et al. 1986. Science 232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
Brodeur, G.M., et al. 1984. Science 224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
Kohl, N.E., et al. 1983. Cell 35, 603.
Schwab, M., et al. 1983. Nature 305, 245.

Citations

Title
  • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    		•
    Data Sheet

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision02-October-2007 RFH
    ApplicationFrozen Sections (1-5 µg/ml)
    Immunoblotting (1-5 µg/ml, see application references)
    Immunofluorescence (1-5 µg/ml, see application references)
    Immunoprecipitation (1-2 µg/sample, see application references)
    DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.
    BackgroundThe v-myc oncogene, initially identified in the MC29 avian retrovirus, causes myelocytomas, carcinomas, sarcomas and lymphomas, and belongs to a family of oncogenes conserved throughout evolution. In humans the family consists of five genes: c-myc, N-myc, R-myc, L-myc and B-myc. Amplification of the N-myc gene has been found in human neuroblastomas and cell lines. The extent of N-myc amplification correlates well with the stage of neuroblastoma disease. Immunological studies have shown that the human N-myc gene gives rise to at least two nuclear phosphoproteins of ~64 kDa and ~67 kDa that exhibit relatively short (30 min) half lives in vivo and exhibit DNA binding properties in vitro.
    HostMouse
    Immunogen speciesHuman
    Immunogenrecombinant, human, N-myc fusion protein, expressed in E. coli
    CloneNCM II 100
    IsotypeIgG₁
    Specieshuman, mouse
    Positive controlIMR5 cells
    FormLiquid
    FormulationIn 50 mM sodium phosphate buffer, 50% glycerol.
    Concentration Label Please refer to vial label for lot-specific concentration
    PreservativeNone
    CommentsIt is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.
    Storage Avoid freeze/thaw
    -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    ReferencesBrondyk, W.H. 1991. Oncogene 6, 1269.
    Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research. 2, 133.
    LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
    Cole, M.D., 1986. Ann. Rev. Gen. 20, 361.
    Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929.
    Slamon, D.J., et al. 1986. Science 232, 768.
    Seeger, R.C., et al. 1985. New Engl. J. Med. 313, 1111.
    Brodeur, G.M., et al. 1984. Science 224, 1121.
    Michitsch, R.W., et al. 1984. Mol. Cell. Biol. 11, 2370.
    Kohl, N.E., et al. 1983. Cell 35, 603.
    Schwab, M., et al. 1983. Nature 305, 245.
    Citation
  • Hongjuan Cui, Tai Li and Han-Fei Ding. (2005) Linking of n-myc to death receptor machinery in neuroblastoma cells. Journal of Biological Chemistry 280, 9474-9481.
    		•
    Application referencesOriginal Clone, Immunofluorescence Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoblotting Brondyk, W.H. 1991. Oncogene 6, 1269. Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 5929. Immunoprecipitation Brondyk, W.H. 1991. Oncogene 6, 1269.