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ABE413-25UG Anti-NF-E2-related factor 2 (rabbit polyclonal)

Anti-NF-E2-related factor 2 Antibody is a highly specific rabbit polyclonal antibody, that targets NF-E2-related factor 2 & has been tested in western blotting & ICC.

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ABE413-25UG
25 μg  
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Description
Catalogue NumberABE413-25UG
DescriptionAnti-NF-E2-related factor 2 (rabbit polyclonal)
Alternate Names
  • Nuclear factor erythroid 2-related factor 2
  • NF-E2-related factor 2
  • NFE2-related factor 2
  • HEBP1
  • Nuclear factor erythroid derived 2-like 2
Background InformationNF-E2-related factor 2 (Nuclear factor erythroid 2-related factor 2) is a transcription activator that binds to antioxidant response elements (ARE) in the promoter regions of target genes. NF-E2-related factor 2 mediates the coordinated up-regulation of genes in response to oxidative stress. NF-E2-related factor 2 may also be involved in the transcriptional activation of genes of the beta-globin cluster. NF-E2-related factor 2 is widely expressed, with highest expression in adult muscle, kidney, lung, liver and fetal muscle.
References
Product Information
FormatAffinity Purified
Control
  • Ramos cell lysate
PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NF-E2-related factor 2 Antibody is a highly specific rabbit polyclonal antibody, that targets NF-E2-related factor 2 & has been tested in western blotting & ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected NF-E2 related factor 2 in 10 µg of Jurkat cell lysate.

Immunocytochemistry Analysis: A 1:200 dilution from a representative lot detected NF-E2 related factor 2 in cells untreated and treated with hydrogen peroxide (Note: Cytoplasmic staining is increased under oxidative stress.).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of human NF-E2 related factor 2.
EpitopeC-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis antibody recognizes the C-terminus of NF-E2 related factor 2
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Chimpanzee
  • Rhesus Macaque
Species Reactivity NoteDemonstrated to react with Human. Predicted to react with Mouse, Rat, Chimpanzee, and Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NFE2L2
  • NRF2
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~75 kDa observed. Two isoforms at 68 kDa (isoform 1) and 66 kDa (isoform 2) are known to exist. This protein has been observed at ~75 kDa due to the abundance of acidic residues found in Nrf2, which causes it to migrate anomalously in SDS-PAGE (Abu-Bakar, A., et al. (2007) Drug Metab Dispos. 35(5):787-794.). A ubiquitinated form of NF-E2-related factor 2 may be observed at ~110 kDa (Li, J., et al. (2005). Toxicol Sci. 83(2):313-328.).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Ramos cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected NF-E2 related factor 2 in 10 µg of Ramos cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE413-25UG 04054839342592

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