Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

04-804 Anti-NFAT1 Antibody, clone 8G4.2

This Anti-NFAT1 Antibody, clone 8G4.2 is validated for use in WB, IF for the detection of NFAT1.

Anti-NFAT1 Antibody, clone 8G4.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Transcription Factors Gene Symbol: KIAA0611, NF-ATP , NF-ATp , NFAT1, NFATP, NFATp UniProt Number: Q13469
View Products on Sigmaaldrich.com
04-804
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, IFMPurifiedMonoclonal Antibody
Description
Catalogue Number04-804
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-NFAT1 Antibody, clone 8G4.2
Alternate Names
  • Nuclear factor of activated T-cells, cytoplasmic 2
  • NF-ATc2
  • NFATc2
  • NFAT pre-existing subunit
  • NF-Atp
  • T-cell transcription factor NFAT1
Background InformationNFAT (Nuclear Factor of Activated T-cells) is a family of transcription factors that are located in the cytoplasm in their inactive state. NFAT translocates to the nucleus upon stimulation of the T-cell receptor where it plays a key role in gene expression in immune response. NFAT is dephosphorylated and activated by the phosphatase PP2B/Calcineurin. This results in its nuclear localization and transcriptional activity. For NFAT1 (NFATC2, nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2), calcineurin is known to target the region between residues 114-126. Mutations in this region of NFAT1 result in its inability to de-phosphorylate and subsequent nuclear translocation. Upon activation, NFAT mediates the transcriptional induction of cytokines and other immuno-regulatory genes during immune responses, and is expressed in T-cells, B-cells, mast cells and NK cells.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal in PBS with 0.05% NaN3.
Quality LevelMQ100
Applications
ApplicationThis Anti-NFAT1 Antibody, clone 8G4.2 is validated for use in WB, IF for the detection of NFAT1.
Key Applications
  • Western Blotting
  • Immunofluorescence
Biological Information
ImmunogenGST-fusion protein corresponding to amino acids 1-297 of human NFAT1.
EpitopeN-term
Clone8G4.2
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityDetects NFAT1.
IsotypeIgMκ
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, and Rat. Other species have not been tested.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThis gene is a member of the nuclear factor of activated T cells (NFAT) family. The product of this gene is a DNA-binding protein with a REL-homology region (RHR) and an NFAT-homology region (NHR). This protein is present in the cytosol and only translocates to the nucleus upon T cell receptor (TCR) stimulation, where it becomes a member of the nuclear factors of activated T cells transcription complex. This complex plays a central role in inducing gene transcription during the immune response. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]
Gene Symbol
  • KIAA0611
  • NF-ATP
  • NF-ATp
  • NFAT1
  • NFATP
  • NFATp
UniProt Number
UniProt SummaryFUNCTION: Plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF.
SUBUNIT: Member of the multicomponent NFATC transcription complex that consists of at least two components, a pre-existing cytoplasmic component NFATC2 and an inducible nuclear component NFATC1. Other members such as NFATC4, NFATC3 or members of the activating protein-1 family, MAF, GATA4 and Cbp/p300 can also bind the complex. The phosphorylated form specifically interacts with XPO1; which mediates nuclear export. NFATC proteins bind to DNA as monomers. Interacts with NFATC2IP (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC play a key role in the gene transcription.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.
TISSUE SPECIFICITY: Expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas.
INDUCTION: Inducibly expressed in T-lymphocytes upon activation of the T-cell receptor (TCR) complex. Induced after co-addition of phorbol 12-myristate 13-acetate (PMA) and ionomycin.
DOMAIN: Rel Similarity Domain (RSD) allows DNA-binding and cooperative interactions with AP1 factors (By similarity).
PTM: In resting cells, phosphorylated by NFATC-kinase on at least 18 sites in the 99-363 region. Upon cell stimulation, all these sites except Ser-243 are dephosphorylated by calcineurin. Dephosphorylation induces a conformational change that simultaneously exposes an NLS and masks an NES, which results in nuclear localization. Simultaneously, Ser-53 or Ser-56 is phosphorylated; which is required for full transcriptional activity.
SIMILARITY: Contains 1 RHD (Rel-like) domain.
Molecular Weight120-140 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by Western Blot
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-804 04053252619243

Documentation

Anti-NFAT1 Antibody, clone 8G4.2 SDS

Title

Safety Data Sheet (SDS) 

Anti-NFAT1 Antibody, clone 8G4.2 Certificates of Analysis

TitleLot Number
Anti-NFAT1, clone 8G4.2 - 2172755 2172755
Anti-NFAT1, clone 8G4.2 - 3501840 3501840
Anti-NFAT1, clone 8G4.2 - 3738074 3738074
Anti-NFAT1, clone 8G4.2 - JH1769963 JH1769963
Anti-NFAT1, clone 8G4.2 - NG1547146 NG1547146
Anti-NFAT1, clone 8G4.2 - NG1631262 NG1631262
Anti-NFAT1, clone 8G4.2 - NG1873194 NG1873194
Anti-NFAT1, clone 8G4.2 -2499300 2499300
Anti-NFAT1, clone 8G4.2 -2741546 2741546
Anti-NFAT1, clone 8G4.2 -2748011 2748011
Show more