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MAB5384A4 Anti-NG2 Antibody, clone 132.39, Alexa Fluor®488 Conjugate

Anti-NG2 Antibody, clone 132.39, Alexa Fluor®488 Conjugate detects level of NG2 & has been published & validated for use in ICC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: Cspg4, Ng2, NG2 UniProt Number: Q00657
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MAB5384A4
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMAB5384A4
DescriptionAnti-NG2 Antibody, clone 132.39, Alexa Fluor®488 Conjugate
Alternate Names
  • Chondroitin sulfate proteoglycan 4
  • Chondroitin sulfate proteoglycan NG2
  • HSN tumor-specific antigen
Background InformationNG2 is a high molecular weight, integral membrane chondroitin sulfate proteoglycan. It is found on the surfaces of an unusual class of glial cells within the developing and mature central nervous system that have the properties of oligodendrocyte precursor cells (i.e., O-2A progenitor cells). NG2 is also found on the surfaces of chondroblasts, proliferating capillary endothelial cells, some human melanoma cell lines, and on leukemic blasts in childhood acute lymphoblastic leukemia. The NG2 proteoglycan is likely to play a role in regulation of cell motility, axon outgrowth and the cellular responses to platelet-derived growth factor.
References
Product Information
FormatAlexaFluor®488
Control
  • Rat oligodendrocyte precursor cells
PresentationPurified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationAnti-NG2 Antibody, clone 132.39, Alexa Fluor®488 Conjugate detects level of NG2 & has been published & validated for use in ICC.
Key Applications
  • Immunocytochemistry
Biological Information
ImmunogenCell line expressing a truncated form of NG2
Clone132.39
HostMouse
SpecificityThis antibody recognizes the native, recombinant, and protein from cells which express NG2.
IsotypeIgG1
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteDemonstrated to react with Rat. Expected to react with Mouse and Human based on sequence similarty.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryExtracellular matrix protein secreted by many cell types; member of the large chondroitin sulfate proteoglycan (CSPG) family; may be involved with angiogenesis -dependent processes such as wound healing or tumor growth [RGD].
Gene Symbol
  • Cspg4
  • Ng2
  • NG2
Purification MethodProtein G Purified
UniProt Number
UniProt SummaryFUNCTION: Proteoglycan playing a role in cell proliferation and migration which stimulates endothelial cells motility during microvascular morphogenesis. May also inhibit neurite outgrowth and growth cone collapse during axon regeneration. Cell surface receptor for collagen alpha 2(VI) which may confer cells ability to migrate on that substrate. Binds through its extracellular N- terminus growth factors, extracellular matrix proteases modulating their activity. May regulate MPP16-dependent degradation and invasion of type I collagen participating in melanoma cells invasion properties. May modulate the plasminogen system by enhancing plasminogen activation and inhibiting angiostatin. Functions also as a signal transducing protein by binding through its cytoplasmic C-terminus scaffolding and signaling proteins. May promote retraction fiber formation and cell polarization through Rho GTPase activation. May stimulate alpha-4, beta-1 integrin- mediated adhesion and spreading by recruiting and activating a signaling cascade through CDC42, ACK1 and BCAR1. May activate FAK and ERK1/ERK2 signaling cascades.

SIZE: 2326 amino acids; 251909 Da

SUBUNIT: Interacts with GRIP1, GRIP2 and GRIA2. Forms a ternary complex with GRIP1 and GRIA2. Interacts with ITGA4 through its chondroitin sulfate glycosaminoglycan. Interacts with BCAR1, CDC42 and ACK1. Interacts with MMP16 (By similarity). Interacts with the first PDZ domain of MPDZ. Interacts with PRKCA. Interacts with LGALS3 and the integrin composed of ITGB1 and ITGA3. Binds TNC, laminin-1, COL5A1 and COL6A2. Interacts with PLG and angiostatin. Binds FGF2 and PDGFA.

SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein; Extracellular side. Apical cell membrane; Single-pass type I membrane protein; Extracellular side. Cell projection › lamellipodium membrane; Single-pass type I membrane protein; Extracellular side. Note= Localizes to the plasma membrane of oligodendrocytes (By similarity). Localized at the apical plasma membrane it relocalizes to the lamellipodia of astrocytoma upon phosphorylation by PRKCA. Localizes to the retraction fibers. A fraction may undergo cell surface proteolysis and secretion.

TISSUE SPECIFICITY: Neural cells and also extraneural tissues, especially in the developing mesenchyme.

PTM: N-glycosylated (By similarity). & O-glycosylated; contains glycosaminoglycan chondroitin sulfate which are required for proper localization and function in stress fiber formation. Involved in interaction with MMP16 and ITGA4. Phosphorylation by PRKCA regulates its subcellular location and function in cell motility.

MISCELLANEOUS: Valuable marker for several incompletely differentiated precursor cells.
Molecular WeightThe unconjugated parent antibody (Cat. No. MAB5384) has an observed molecular weight at ~280 kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in rat oligodendrocyte precursor cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected NG2 in rat oligodendrocyte precursor cells. Performed without Triton.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB5384A4 04053252647758

Documentation

Anti-NG2 Antibody, clone 132.39, Alexa Fluor®488 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-NG2 Antibody, clone 132.39, Alexa Fluor®488 Conjugate Certificates of Analysis

TitleLot Number
Anti-NG2, clone 132.39, Alexa -2738999 2738999
Anti-NG2, clone 132.39, Alexa Fluor#174;488 Conjugate - 2204994 2204994
Anti-NG2, clone 132.39, Alexa Fluor#174;488 Conjugate - Q2018166 Q2018166
Anti-NG2, clone 132.39, Alexa Fluor®488 Conjugate 2982451
Anti-NG2, clone 132.39, Alexa Fluor®488 Conjugate 3013402
Anti-NG2, clone 132.39, Alexa Fluor®488 Conjugate 2939482
Anti-NG2, clone 132.39, Alexa Fluor®488 Conjugate 2869047
Anti-NG2, clone 132.39, Alexa Fluor®488 Conjugate 3109196
Anti-NG2, clone 132.39, Alexa Fluor®488 Conjugate - 2458013 2458013
Anti-NG2, clone 132.39, Alexa Fluor®488 Conjugate - 2294356 2294356
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies