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MABF1032 Anti-NKG2D (CD314) Antibody, clone 1D11

Anti-NKG2D (CD314), clone 1D11, Cat. No. MABF1032, is a mouse monoclonal antibody that detects human NKG2-D type II integral membrane protein and has been tested for use in Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry, Immunoprecipitation, ECM Adhesion and Inhibit

Anti-NKG2D (CD314) Antibody, clone 1D11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: KLRK1, D12S2489E, NKG2D UniProt Number: P26718
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MABF1032
200 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, FACS, IHC, IP, INHIBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF1032
DescriptionAnti-NKG2D (CD314) Antibody, clone 1D11
Alternate Names
  • NKG2-D type II integral membrane protein
  • Killer cell lectin-like receptor subfamily K member 1
  • NK cell receptor D
  • NKG2-D-activating NK receptor
  • CD314
Background InformationNKG2-D type II integral membrane protein (UniProt: P26718; also known as Killer cell lectin-like receptor subfamily K member 1, NK cell receptor D, NKG2-D-activating NK receptor, CD314) is encoded by the KLRK1 (also known as D12S2489E, NKG2D) gene (Gene ID: 100528032) in human. NK cell receptor D is a homodimeric, disulfide-linked, single-pass type II membrane protein that can also form heterohexamers along with four subunits of HCST/DAP10. NK cell receptor D contains a cytoplasmic domain (aa 1-51), a transmembrane helical domain (aa 52-72), and an extracellular domain (aa 73-216). It functions as an activating and co-stimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. It provides both stimulatory and co-stimulatory innate immune responses on activated killer (NK) cells, leading to their cytotoxic activity. Also acts as a co-stimulatory receptor for T-cell receptor (TCR) in CD8+ T-cell-mediated adaptive immune responses by amplifying T-cell activation. NK cell receptor D is expressed in natural killer (NK) cells, CD8+ alpha-beta and gamma-delta T-cells. Its expression in NK cells is upregulated by interleukin 15 (IL15).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-NKG2D (CD314), clone 1D11, Cat. No. MABF1032, is a mouse monoclonal antibody that detects human NKG2-D type II integral membrane protein and has been tested for use in Flow Cytometry, Fluorescence Activated Cell Sorting (FACS), Immunohistochemistry, Immunoprecipitation, ECM Adhesion and Inhibit
Key Applications
  • Flow Cytometry
  • Fluorescence Activated Cell Sorting (FACS)
  • Immunohistochemistry
  • Immunoprecipitation
  • Inhibits Activity/Function
Application NotesFluorescence Activated Cell Sorting (FACS): A representative lot detected NKG2D (CD314) in Fluorescence Activated Cell Sorting applications (Groh, V., et. al. (2003). Proc Natl Acad Sci U S A. 100(16):9452-7).

Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected NKG2D (CD314) in CD3-/CD56+ T cells (Courtesy of Alejandro Lopez-Soto, Ph.D., Tumor Immunology Group, Universidad de Oviedo, IUOPA Spain).

Inhibits Activity/Function: A representative lot of Anti-NKG2D (CD314), clone 1D11, reduced the percentage of degranulating NK cells in freshly isolated PBMCs when co-incubated with K562 cells (Courtesy of Alejandro Lopez-Soto, Ph.D., Tumor Immunology Group, Universidad de Oviedo, IUOPA Spain).

Immunohistochemistry Analysis: A representative lot detected NKG2D (CD314) in Immunohistochemistry applications (Groh, V., et. al. (2003). Proc Natl Acad Sci U S A. 100(16):9452-7).

Inhibition Analysis: A representative lot inhibited NKG2D activated cytolytic responses of gamma delta T cells and NK cells against transfectants and epithelial tumor cells expressing MICA. (Bauer, S., et. al. (1999). Science. 285(5428):727-9).

Stimulates ECM Adhesion: A representative lot of Anti-NKG2D (CD314), clone 1D11 stimulated ECM Adhesion (Abeyweera, T.P., et. al. (2011). J Cell Biol. 192(4):675-90).

Flow Cytometry Analysis: A representative lot detected NKG2D (CD314) in Flow Cytometry applications (Wu, J., et. al. (2002). J Immunol. 169(3):1236-40; Bauer, S., et. al. (1999). Science. 285(5428):727-9; Wu, J., et. al. (1999). Science. 285(5428):730-2; Roberts, A.I., et. al. (2001). J Immunol. 167(10):5527-30).

Immunoprecipitation Analysis: A representative lot detected NKG2D (CD314) in Immunoprecipitation applications (Bauer, S., et. al. (1999). Science. 285(5428):727-9).
Biological Information
ImmunogenWhole NKL cells (aggressive cell line derived from human natural killer cell leukemia).
Clone1D11
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 1D11 detects NKG2-D type II integral membrane protein in human cells.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • KLRK1
  • D12S2489E
  • NKG2D
Purification MethodProtein G purified
UniProt Number
Molecular Weight25.27 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceIsotype testing: Identity Confirmation by Isotyping Test.

Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF1032 04054839369957