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MABF2241-100UL Anti-NKp30 Antibody, clone 30.95.1

Anti-NKp30, clone 30.95.1, Cat. No. MABF2241, is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30) and is tested for use in Flow Cytometry.

MABF2241-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF2241-100UL
DescriptionAnti-NKp30 Antibody, clone 30.95.1
Alternate Names
  • Natural cytotoxicity triggering receptor 3
  • Activating natural killer receptor p30
  • Natural killer cell p30-related protein
  • NK-p30
  • CD337
Background InformationNatural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane protein that is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of natural killer/NK cell. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1. It stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF-alpha and interferon-gamma by NK cells that promote DC maturation.NKp30 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). Six isoforms of NKp30 have been described that are produced by alternative splicing.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Applications
ApplicationAnti-NKp30, clone 30.95.1, Cat. No. MABF2241, is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30) and is tested for use in Flow Cytometry.
Key Applications
  • Flow Cytometry
Application NotesFlow Cytometry Analysis: A representative lot detected NKp30 in Flow Cytometry applications (Barrow, A.D., et. al. (2018). Cell. 172(3):534-548).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHuman NK cells.
Epitopeextracellular domain
Clone30.95.1
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 30.95.1 is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30). It targets an epitope within the extracellular domain.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NCR3
  • IC7
  • LY117
Purification MethodProtein G purified
UniProt Number
Molecular Weight21.59 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in NK-92 cells.

Flow Cytometry Analysis: 1 µg of this antibody detected NKp30 in one million NK-92 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2241-100UL 04061841844471

Documentation

Anti-NKp30 Antibody, clone 30.95.1 SDS

Title

Safety Data Sheet (SDS) 

Anti-NKp30 Antibody, clone 30.95.1 Certificates of Analysis

TitleLot Number
Anti-NKp30, clone 30.95.1 - Q3438663 Q3438663

References

Reference overviewPub Med ID
Natural Killer Cells Control Tumor Growth by Sensing a Growth Factor
Alexander D Barrow 1 , Melissa A Edeling 1 , Vladimir Trifonov 2 , Jingqin Luo 3 , Piyush Goyal 2 , Benjamin Bohl 2 , Jennifer K Bando 1 , Albert H Kim 4 , John Walker 2 , Mary Andahazy 2 , Mattia Bugatti 5 , Laura Melocchi 5 , William Vermi 6 , Daved H Fremont 1 , Sarah Cox 2 , Marina Cella 1 , Christian Schmedt 2 , Marco Colonna
Cell  172(3)  534-548  2018

Show Abstract 		29275861 29275861