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MABF3113-100UG Anti-NKp30/CD337 Antibody, clone F252

Anti-NKp30/CD337, clone F252, Cat. No. MABF3113. is a mouse monoclonal antibody that detects -NKp30/CD337 and is tested for use in Flow Cytometry, Neutralizing, and Inhibition.

NewMABF3113-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF3113-100UG
DescriptionAnti-NKp30/CD337 Antibody, clone F252
Alternate Names
  • Natural cytotoxicity triggering receptor 3
  • Activating natural killer receptor p30
  • Natural killer cell p30-related protein
  • NK-p30
  • CD337
Background InformationNatural cytotoxicity triggering receptor 3 (UniProt: O14931; also known as Activating natural killer receptor p30, Natural killer cell p30-related protein, NK-p30, NKp30, CD337) is encoded by the NCR3 (also known as 1C7, LY117) gene (Gene ID: 259197) in human. NKp30 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 19-135), a transmembrane domain (aa 136-156), and a cytoplasmic domain (aa 157-201). It is selectively expressed by all resting and activated NK cells. Its weaker expression is observed in spleen. It serves as a receptor of NK cells. It is activated by binding of extracellular ligands including BAG6 and NCR3LG1 and stimulates NK cells cytotoxicity toward neighboring cells producing these ligands. It cooperates with NKp46 and/or NKp44 in inducing NK-mediated cytotoxicity against the majority of target cells. It is also reported to control NK cells cytotoxicity against tumor cells. Its interaction with BAG6 promotes myeloid dendritic cells (DC) maturation, both through killing DCs that fail to achieve a mature phenotype and by inducing the release of TNF- and interferon- by NK cells that promote DC maturation. Masking of NKp30 with specific monoclonal antibodies is reported to reduce maturation of DCs cocultured with NK cells. Addition of supernatant from NK cells stimulated via NKp30 is shown to induce DC maturation. Six isoforms of NKp30 have been described that are produced by alternative splicing. (Ref.: Vitale, M., et al. (2005). Blood. 106(2); 566-571; Pende, D., et al. (1999). J. Exp. Med. 190(10); 1505-1516).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgM in PBS without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-NKp30/CD337, clone F252, Cat. No. MABF3113. is a mouse monoclonal antibody that detects -NKp30/CD337 and is tested for use in Flow Cytometry, Neutralizing, and Inhibition.
Key Applications
  • Flow Cytometry
  • Affects Function
  • Neutralizing
  • Inhibition
Application NotesTested Applications

Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Flow Cytometry application. (Meazza, R., et al. (2020). Cancers (Basel). 12(8):2187; Salome, B., et al. (2022). Cancer Cell. 40(9):1027-1043.e9).

Inhibition: A representative lot Inhibited the lysis of antigen presenting cells (APC) and blocked the expression of CD83, CD86.(Spaggiari, G.M., et al. (2001). Eur J Immunol.;31(6):1656-65; Vitale, M., et al. (2005). Blood. 106(2):566-71).

Flow Cytometry Analysis: A representative lot detected NKp30 (CD337) in Human peripheral blood mononuclear cells (PBMC). (Courtesy of Dr. Raffaella Meazza, IRCCS Ospedale Policlinico San Martino, Genova, Italy).

Neutralizing: A representative lot neutralized the receptor function of NKp30 (CD337). (Witkowski, M., et al. (2021). Nature. 600(7888):295-301).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenActivated human natural killer (NK) cells.
EpitopeExtracellular domain
CloneF252
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone F252 is a mouse monoclonal antibody that detects Natural cytotoxicity triggering receptor 3 (NKp30/CD337). It targets an epitope within the extracellular domain.
IsotypeIgMκ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NCR3
Purification MethodLigatrap
UniProt Number
Molecular Weight21.59 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in Human peripheral blood mononuclear cells (PBMC).

Flow Cytometry Analysis: 1 µg of this antibody detected NKp30 in one million Human peripheral blood mononuclear cells (PBMC).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF3113-100UG 04065271175129