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AB5048P Anti-NMDAR1 Splice Variant C2 Antibody

This Anti-NMDAR1 Splice Variant C2 Antibody is validated for use in IH, WB for the detection of NMDAR1 Splice Variant C2.

Anti-NMDAR1 Splice Variant C2 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: GRIN1, Glutamate, NMDAR1, NMDA1, NR1 UniProt Number: Q05586
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AB5048P
25 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, RIHC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5048P
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-NMDAR1 Splice Variant C2 Antibody
References
Product Information
FormatAffinity Purified
PresentationAffinity purified immunoglobulin. Lyophilized from ammonium bicarbonate (5 mM) so some residual salt may also be present. Reconstitute with 50 μL of PBS. Contains no preservatives.
Quality LevelMQ100
Applications
ApplicationThis Anti-NMDAR1 Splice Variant C2 Antibody is validated for use in IH, WB for the detection of NMDAR1 Splice Variant C2.
Key Applications
  • Immunohistochemistry
  • Western Blotting
Application NotesWestern blot: 1:1000 using ECL

Immunohistochemistry: 1:1,000

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenPeptide corresponding to the C-terminus of the rat NMDAR1 C2 splice variant.
HostRabbit
SpecificitySpecific for the NMDAR1 C2 splice variants. Recognizes NMDAR-1a, 1b, 2a, and 2b NR1 splice variants. {Gorter et al, (1997), "The Role of Alternative Splicing of the NMDAR1 Receptor Subunit in Synaptic Plasticity" Chapter 4, pg 99, in The Ionotropic Glutamate Receptors , Monaghan, D.T. and Weinhold, R.J. (eds), Humana Press, New Jersey) By Western blot the antibody recognizes a protein with molecular weight of 120 kDa.
Species Reactivity
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a critical subunit of N-methyl-D-aspartate receptors, members of the glutamate receptor channel superfamily which are heteromeric protein complexes with multiple subunits arranged to form a ligand-gated ion channel. These subunits play a key role in the plasticity of synapses, which is believed to underlie memory and learning. Cell-specific factors are thought to control expression of different isoforms, possibly contributing to the functional diversity of the subunits. Alternatively spliced transcript variants have been described.
Gene Symbol
  • GRIN1
  • Glutamate
  • NMDAR1
  • NMDA1
  • NR1
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q05586 # NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors (By similarity).
SIZE: 938 amino acids; 105373 Da
SUBUNIT: Forms heteromeric channel of a zeta subunit (GRIN1), a epsilon subunit (GRIN2A, GRIN2B, GRIN2C or GRIN2D) and a third subunit (GRIN3A or GRIN3B); disulfide-linked. Found in a complex with GRIN2A or GRIN2B, GRIN3A or GRIN3B and PPP2CB. Interacts with DLG4 and MPDZ (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein (By similarity). Note=Enriched in post-synaptic plasma membrane and post-synaptic densities (By similarity).
PTM: NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity (By similarity).
SIMILARITY: SwissProt: Q05586 ## Belongs to the glutamate-gated ion channel (TC 1.A.10) family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size25 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5048P 04053252509445

Documentation

Anti-NMDAR1 Splice Variant C2 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-NMDAR1 Splice Variant C2 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2426491 2426491
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2458962 2458962
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT - 4049856 4049856
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY 2953860
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY 2961763
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY 2984186
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY 2919751
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY 3002840
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY 3143225
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2193283 2193283

References

Reference overviewPub Med ID
Heteromeric NMDA receptors: molecular and functional distinction of subtypes.
Monyer, H, et al.
Science, 256: 1217-21 (1992)  1992

Show Abstract 		1350383 1350383

Brochure

Title
Pathways and Biomarkers of Glutamatergic Synapse Flyer

Data Sheet

Title
RABBIT ANTI-NMDAR1 C2 SPLICE VARIANT AFFINITY PURIFIED POLYCLONAL ANTIBODY

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies