Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABE2867-25UL Anti-NR5A2 (LRH1)

Anti-NR5A2 (LRH1), Cat. No. ABE2867-25UL, is a highly specific rabbit polyclonal antibody, that targets Nuclear receptor subfamily 5 group A member 2 and has been tested in Western Blotting, Immunofluorescence and Chromatin Immunoprecipitation (ChIP).

Anti-NR5A2 (LRH1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: NR5A2, B1F, CPF, FTF UniProt Number: O00482
View Products on Sigmaaldrich.com
ABE2867-25UL
25 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, IF, ChIPRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABE2867-25UL
DescriptionAnti-NR5A2 (LRH1)
Alternate Names
  • Nuclear receptor subfamily 5 group A member 2
  • Alpha-1-fetoprotein transcription factor
  • B1-binding factor
  • hB1F
  • CYP7A promoter-binding factor
  • Hepatocytic transcription factor
  • Liver receptor homolog 1
  • LRH-1
Background InformationNuclear receptor subfamily 5 group A member 2 (UniProt: O00482; also known as Alpha-1-fetoprotein transcription factor, B1-binding factor, hB1F, CYP7A promoter-binding factor, Hepatocytic transcription factor, Liver receptor homolog 1, LRH-1) is encoded by the NR5A2 (also known as B1F, CPF, FTF) gene (Gene ID: 2494) in human. It is a member of the nuclear receptor superfamily that binds to the sequence element 5'-AACGACCGACCTTGAG-3' of the enhancer II of hepatitis B virus genes. It contains a DNA binding region (aa 83-104) and two NR C4-type zinc finger domains (aa 86-106 and 122-146). It is abundantly expressed in pancreas, but at reduced levels in liver, and at very low levels in heart and lung. It s expression has been demonstrated in the Hep-G2 cell line. Four different isoforms of LRH-1 have been described that are generated by alternate splicing. LRH-1 is reported to be involved in the regulation of expression of transcription factors, such as Oct4, HNF1, 3, and 4, which are known to participate in embryonic development. Its presence in high levels in beta-III Tubulin+ and NeuN+ neurons of the mantle zone (MZ) in early neural tube of mice (E10.5 and E12.5) indicate its involvement in neural differentiation. (Ref.: Stergiopoulos, A., and Politis, PK (2016). Nat. Commun. DOI: 10.1038/ncomms12230; Venteclef, N et al. (2008). J. Biol. Chem. 283(7); 3694 3701).
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-NR5A2 (LRH1), Cat. No. ABE2867-25UL, is a highly specific rabbit polyclonal antibody, that targets Nuclear receptor subfamily 5 group A member 2 and has been tested in Western Blotting, Immunofluorescence and Chromatin Immunoprecipitation (ChIP).
Key Applications
  • Western Blotting
  • Immunofluorescence
  • Chromatin Immunoprecipitation (ChIP)
Application NotesChromatin Immunoprecipitation Analysis: A representative lot detected NR5A2 (LRH1) in Chromatin Immunoprecipitation applications (Kaltezioti, V., et. al. (2010). PLoS Biol. 8(12):e1000565; Venteclef, N., et. al. (2010). Genes Dev. 24(4):381-95; Boulias, K., et. al. (2005). EMBO J. 24(14):2624-33; Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105).

Western Blotting Analysis: A representative lot detected NR5A2 (LRH1) in Western Blotting applications (Chalkiadaki, A., et. al. (2005). Mol Cell Biol. 25(12):5095-105; Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).

Immunofluorescence Analysis: A representative lot detected NR5A2 (LRH1) in Immunofluorescence YES titration paraffin sections from E10.5 (a), E12.5 (b), E14.5 (c) or E16.5 (d) mouse spinal cord (Stergiopoulos, A., et. al. (2016). Nat Commun. 7:12230).
Biological Information
ImmunogenHis-tagged full length recombinant human Nuclear receptor subfamily 5 group A member 2, isoform 2.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Nuclear receptor subfamily 5 group A member 2 (NR5A2) in human and murine cells.
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse
Antibody TypePolyclonal Antibody
Gene Symbol
  • NR5A2
  • B1F
  • CPF
  • FTF
Purification MethodUnpurified
UniProt Number
Molecular Weight68 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected NR5A2 (LRH1) in 10 µg of HEK293 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE2867-25UL 04054839474910