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MABE995 Anti-NRF-1 Antibody, clone R157.1.3H3

This Anti-NRF-1 Antibody, clone R157.1.3H3 is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of NRF-1.

Anti-NRF-1 Antibody, clone R157.1.3H3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NRF1 UniProt Number: Q16656
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MABE995
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ChIP, ChIP-seqMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE995
DescriptionAnti-NRF-1 Antibody, clone R157.1.3H3
Alternate Names
  • Nuclear respiratory factor 1
  • NRF-1
  • Alpha palindromic-binding protein
  • Alpha-pal
  • NRF-1
  • Nuclear respiratory factor 1
Background InformationNuclear respiratory factor 1 (UniProt Q16656; also known as Alpha-pal, Alpha palindromic-binding protein, NRF-1) is encoded by the NRF1 (also known as ALPHA-PAL) gene (Gene ID 4899) in human. NRF-1 is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. NRF-1 is upregulated and activated/phosphorylated by Akt in response to oxidant exposure or increased cellular reactive oxygen species (ROS), resulting in NRF-1 nuclear translocation, where it binds to DNA as a homodimer and mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-NRF-1 Antibody, clone R157.1.3H3 is validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of NRF-1.
Key Applications
  • Western Blotting
  • Chromatin Immunoprecipitation (ChIP)
  • ChIP-seq
Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected NRF-1 in 10 µg of SH-SY5Y and HeLa cell lysates.
Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected NRF-1 chromatin DNA binding activity using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).
ChIP-seq Analysis: A representative lot detected NRF-1 DNA binding motifs by ChIP-seq analysis using K562 chromatin preparations (ENCODE Data Coordination Center (DCC) accession: ENCAB000BLM).
Biological Information
ImmunogenRecombinant protein corresponding to human NRF-1.
CloneR157.1.3H3
ConcentrationPlease refer to lot specific datasheet.
HostMouse
IsotypeIgG2aκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NRF1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~58 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected NRF-1 in 10 µg of Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE995 04055977288360