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05-369-25UG Anti-Na+/K+ ATPase a-1 Antibody,clone C464.6

Detect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.

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05-369-25UG
25 μg  
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Description
Catalogue Number05-369-25UG
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Na+/K+ ATPase a-1 Antibody,clone C464.6
Alternate Names
  • ATPase
  • Na+/K+ transporting, alpha 3 polypeptide
  • Na(+)/K(+) ATPase alpha(III) subunit
  • Na(+)/K(+) ATPase alpha-3 subunit
  • Na+/K+ -ATPase alpha 3 subunit
  • Na+/K+ ATPase 3
  • Sodium pump subunit alpha-3
  • dystonia 12
  • sodium pump 3
  • sodium-potassium-ATPase
  • alpha 3 polypeptide
  • sodium/potassium-transporting ATPase alpha-3 chain
Background InformationNa+/K+-ATPase (also known as the Na+/K+ pump or sodium-potassium pump) is an enzyme located in the plasma membrane (specifically an electrogenic transmembrane ATPase). It is found in the plasma membrane of virtually every human cell and is common to all cellular life. It helps maintain cell potential and regulate cellular volume.
In order to maintain the cell potential, cells must keep a low concentration of sodium ions and high levels of potassium ions within the cell (intracellular). Outside cells (extracellular), there are high concentrations of sodium and low concentrations of potassium, so diffusion occurs through ion channels in the plasma membrane. In order to keep the appropriate concentrations, the sodium-potassium pump pumps sodium out and potassium in through active transport.
References
Product Information
FormatPurified
Control
  • Alpha 1 mRNA is present in all cell types examined.
PresentationPurified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine buffer, pH 7.4, 0.15 M NaCl, 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationDetect Na+/K+ ATPase α-1 (sodium/potassium ATPase subunit alpha 1) using this Anti-Na+/K+ ATPase α-1 Antibody, clone C464.6 validated for use in IC, IH, IP & WB.
Key Applications
  • Immunocytochemistry
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation:
2 µg of a previous lot immuno-precipitated the α-1 subunit from rat brain microsomal preparations.

Immunohistochemistry:
1-10 µg/mL of a previous lot of this antibody was used in immunohistochemistry on ice-cold methanol-fixed tissue.

1. Do not boil microsomes. Warming to 37ºC for 15 minutes is recommended.
2. Microsomes from some tissues may contain sodium pump subunits which are below the level of detection by this antibody.
3. Microsomes derived from tissues which are more difficult to prepare, such as smooth and skeletal muscle, frequently show non-specific cross-reactivity.
Biological Information
ImmunogenPurified Na+/K+ ATPase isolated from membrane fractions of rabbit kidney outer medulla.
CloneC464.6
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody recognizes the α-1 subunit isoform of Na+/K+ ATPase.
IsotypeIgG1κ
Species Reactivity
  • Human
  • Rat
  • Rabbit
  • Mouse
  • Monkey
  • Xenopus
  • Pig
  • Canine
  • Sheep
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 3 subunit.
Gene Symbol
  • ATP1A3
  • MGC13276
  • RDP
  • EC 3.6.3.9
  • DYT12
Purification MethodProtein G Chromatography
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P13637 # This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
SIZE: 1013 amino acids; 111749 Da
SUBUNIT: Composed of three subunits: alpha (catalytic), beta and gamma.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DISEASE: SwissProt: P13637 # Defects in ATP1A3 are the cause of dystonia-12 (DYT12) [MIM:128235]; also known as rapid-onset dystonia parkinsonism (RDP). DYT12 is an autosomal dominant form of dystonia- parkinsonism characterized by an unusually rapid evolution of signs and symptoms. Affected persons develop dystonia and parkinsonism between 14 and 45 years of age. The sudden onset of symptoms over hours to a few weeks, often associated with physical or emotional stress, suggests a trigger initiating a nervous system insult resulting in permanent neurologic disability.
SIMILARITY: SwissProt: P13637 ## Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.
Molecular Weight112 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated in western blot on rat brain microsomal preparations.

Western Blot Analysis:
0.05-0.1 µg/mL of this lot detected the α-1 subunit in 20 µg of rat brain microsomal preparation (Catalog # 12-144). To reduce background, wash the blot with 0.05% Tween-20 in PBS for 15 minutes.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-369-25UG 04054839342363

Documentation

Anti-Na+/K+ ATPase a-1 Antibody,clone C464.6 SDS

Title

Safety Data Sheet (SDS) 

Anti-Na+/K+ ATPase a-1 Antibody,clone C464.6 Certificates of Analysis

TitleLot Number
Anti-Na+/K+ ATPase a-1, clone C464.6 - 3185021 3185021
Anti-Na+/K+ ATPase a-1, clone C464.6 - 3215517 3215517
Anti-Na+/K+ ATPase a-1, clone C464.6 - 3277971 3277971
Anti-Na+/K+ ATPase a-1, clone C464.6 - 3484974 3484974
Anti-Na+/K+ ATPase a-1, clone C464.6 - 3828020 3828020
Anti-Na+/K+ ATPase a-1, clone C464.6 - 4061109 4061109
Anti-Na+/K+ ATPase a-1, clone C464.6 - 4097799 4097799
Anti-Na+/K+ ATPase a-1, clone C464.6 - 4181457 4181457

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