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ABE2875 Anti-Nek6

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ABE2875
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, IP, IH(P), IFRbPurifiedPolyclonal Antibody
Description
Catalogue NumberABE2875
DescriptionAnti-Nek6
Alternate Names
  • Serine/threonine-protein kinase Nek6
  • EC: 2.7.11.1
  • Never in mitosis A-related kinase 6
  • NimA-related protein kinase 6
  • Protein kinase SID6-1512
Background InformationSerine/threonine-protein kinase Nek6 (UniProt: Q9HC98; also known as EC: 2.7.11.1, Never in mitosis A-related kinase 6, NimA-related protein kinase 6, Protein kinase SID6-1512) is encoded by the NEK6 gene (Gene ID: 10783) in human. Nek6, a member of the NIMA-related serine/threonine kinase family that plays an important role in mitotic cell cycle progression and is required for chromosome segregation at metaphase-anaphase transition. It localizes to spindle microtubules in metaphase and anaphase and to the mid-body during cytokinesis. Nek6 is ubiquitously expressed, with highest expression in heart and skeletal muscle. Its levels are up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers. Nek6 is shown to be directly phosphorylated by Chk1 and Chk2, suggesting that it is a direct target of the DNA damage checkpoint. It is also phosphorylated upon IR or UV-induced DNA damage. Nek6 is activated by phosphorylation at Ser206. Although expression of Nek6 is tightly controlled in normal cells, this protein is highly overexpressed in various human cancers and regulates tumor progression and its expression and activity levels directly correlate to tumorigenicity of human cancer cells. Inhibition of Nek6 can lead to apoptosis. (Ref.: Nassirpour, R et al. (2010). Mol. Cancer Res. 8(5); 717-28).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nek6, Cat. No. ABE2875, is a highly specific rabbit polyclonal antibody that targets Serine/threonine-protein kinase Nek6 and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunohistochemistry (Paraffin)
  • Immunofluorescence
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nek6 in human pancreas and human cervical cancer tissue.

Immunofluorescence Analysis: A representative lot detected Nek6 in HeLa cells that were transfected with Nek6 siRNA (O'Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90).

Western Blotting Analysis: A representative lot detected Nek6 in IP blot from lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole, HeLa cells were either mock transfected or transfected (O'Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; O'Regan, L., et. al. (2015). J Cell Biol. 209(3):349-58).

Immunoprecipitation Analysis: A representative lot detected Nek6 in lysates prepared from untransfected HEK 293 cells synchronized in S or M phase of the cell cycle with hydroxyurea or nocodazole (O'Regan, L., et. al. (2009). Mol Cell Biol. 29(14):3975-90; O'Regan, L., et. al. (2015). J Cell Biol. 209(3):349-58).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the first 15 amino acids from the N-terminal region of human Serine/threonine-protein kinase Nek6.
EpitopeN-terminus
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Serine/threonine-protein kinase Nek6 in human cells. It targets an epitope with in the first 15 amino acids in the N-terminal region.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NEK6
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~35 kDa observed; 35.71 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HT1080 cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected Nek6 in 10 µg of HT1080 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE2875 04054839315978