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AB5583-50UL Anti-Nerve Growth Factor Antibody, pro

This Anti-Nerve Growth Factor Antibody, pro is validated for use in WB, IH for the detection of Nerve Growth Factor.

Anti-Nerve Growth Factor Antibody, pro MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins Gene Symbol: NTF3, HDNF, NGF-2, NT3, NGF2, MGC129711, NT-3 UniProt Number: P20783
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AB5583-50UL
50 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
RWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5583-50UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nerve Growth Factor Antibody, pro
Alternate Names
  • NGF, pro
References
Product Information
FormatAffinity Purified
Control
  • Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 μL of sterile distilled water. For negative control, preincubate 1 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.025% sodium azide. Reconstitute with 50 mL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Quality LevelMQ100
Applications
ApplicationThis Anti-Nerve Growth Factor Antibody, pro is validated for use in WB, IH for the detection of Nerve Growth Factor.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesWestern blotting: 1:200-1:500 using ECL on His-fusion protein containing the entire prodomain of rat proNGF.

Immunohistochemistry on rat brain sections.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenSynthetic corresponding to amino acids 84-104 of rat proNGF (Accession P25427).
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes proNGF (Nerve Growth Factor).

SPECIES REACTIVITIES:

The immunogen sequence has 100% homology with mouse and 76% homology with human proNGF. Reactivity with other species has not yet been tested.
Species Reactivity
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
Gene Symbol
  • NTF3
  • HDNF
  • NGF-2
  • NT3
  • NGF2
  • MGC129711
  • NT-3
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P20783 # Seems to promotes the survival of visceral and proprioceptive sensory neurons.
SIZE: 257 amino acids; 29355 Da
SUBCELLULAR LOCATION: Secreted.
TISSUE SPECIFICITY: Brain and peripheral tissues.
SIMILARITY: SwissProt: P20783 ## Belongs to the NGF-beta family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5583-50UL 04053252588389

Documentation

Anti-Nerve Growth Factor Antibody, pro SDS

Title

Safety Data Sheet (SDS) 

Anti-Nerve Growth Factor Antibody, pro Certificates of Analysis

TitleLot Number
RABBIT ANTI-proNGF AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2370167 2370167
RABBIT ANTI-proNGF - 3927578 3927578
RABBIT ANTI-proNGF - 4001570 4001570
RABBIT ANTI-proNGF - 4025515 4025515
RABBIT ANTI-proNGF -2589331 2589331
RABBIT ANTI-proNGF -2803222 2803222
RABBIT ANTI-proNGF -2811015 2811015
RABBIT ANTI-proNGF AFFINITY PURIFIED POLYCLONAL ANTIBODY 2947238
RABBIT ANTI-proNGF AFFINITY PURIFIED POLYCLONAL ANTIBODY 3008829
RABBIT ANTI-proNGF AFFINITY PURIFIED POLYCLONAL ANTIBODY 2925711
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