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MAB5386X Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor® 488

This Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor 488 is validated for use in FC, IC for the detection of Nerve Growth Factor Receptor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurochemistry & Neurotrophins Gene Symbol: NGFR, p75(NTR), TNFRSF16, Gp80-LNGFR, CD271 UniProt Number: P08138
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MAB5386X
50 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, Po, Rb, ShFC, ICCMAlexaFluor®488Monoclonal Antibody
Description
Catalogue NumberMAB5386X
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor® 488
Background InformationThe low affinity NGFR (Nerve growth factor receptor) is a 75kDa membrane-spanning glycoprotein lacking intrinsic tyrosine kinase activity. p75NGFR interacts with TrkA, the high affinity NGF receptor and potentiates TrkA signaling at low NGF concentrations. The p75 receptor binds nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4 with varying specificities. The p75NGFR plays an important role in neurotrophic factor signaling and has been shown to modulate the susceptibility of selective cellular populations to programmed cell death. It is expressed on many neuronal cells types including many embryonic forms and the receptor can be used to isolate neuronal progenitor cells
References
Product Information
FormatAlexaFluor®488
PresentationLiquid. Contains no preservative.
Quality LevelMQ100
Applications
ApplicationThis Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor 488 is validated for use in FC, IC for the detection of Nerve Growth Factor Receptor.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
Application NotesOptimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHuman melanoma cell line.
CloneME20.4
HostMouse
SpecificityReacts with the low affinity nerve growth factor receptor (NGFR p75). The epitope recognized by the antibody maps within the third or fourth cysteine-rich repeats of the NGFR molecule. Reactivity has not been observed with rat, chicken, or mouse NGFR.
IsotypeIgG1
Species Reactivity
  • Human
  • Pig
  • Rabbit
  • Sheep
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryNerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain.
Gene Symbol
  • NGFR
  • p75(NTR)
  • TNFRSF16
  • Gp80-LNGFR
  • CD271
Non-Reactive Species
  • Chicken
  • Mouse
  • Rat
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P08138 # Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells.
SIZE: 427 amino acids; 45183 Da
SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR- associated cell death executor. Interacts with TRAF2, TRAF4, TRAF6, PTPN13 and RANBP9. Interacts through TRAF6 with SQSTM1 which bridges NGFR to NTRK1. Interacts with BEX1 and NGFRAP1/BEX3.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DOMAIN: SwissProt: P08138 Death domain is responsible for interaction with RANBP9.
PTM: N- and O-glycosylated. & O-linked glycans consist of Gal(1-3)GalNAc core elongated by 1 or 2 NeuNAc. & Phosphorylated on serine residues.
SIMILARITY: Contains 1 death domain. & Contains 4 TNFR-Cys repeats.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsAliquot and store at -20 ºC, or glycerol (ASC grade or better) can be added (1:1) and the antibody stored at -20ºC for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size50 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB5386X 04053252318269

Documentation

Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor® 488 SDS

Title

Safety Data Sheet (SDS) 

Anti-Nerve Growth Factor Receptor Antibody, p75, Alexa Fluor® 488 Certificates of Analysis

TitleLot Number
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 ALEXA FLUOR 488 CONJUGATED MONOCLONAL ANTIBODY - 2424664 2424664
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 - 3221496 3221496
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 -2759713 2759713
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 ALEXA FLUOR 488 CONJUGATED - 3293710 3293710
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 ALEXA FLUOR#210; 488 CONJUGATED MONOCLONAL ANTIBODY - 2211004 2211004
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 ALEXA FLUOR® 488 CONJUGATED MONOCLONAL ANTIBODY 2975910
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 ALEXA FLUOR® 488 CONJUGATED MONOCLONAL ANTIBODY 3077396
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 ALEXA FLUOR®488 CONJUGATED MONOCLONAL ANTIBODY 3068076

References

Reference overviewPub Med ID
Distribution and co-localization of choline acetyltransferase and p75 neurotrophin receptors in the sheep basal forebrain: implications for the use of a specific cholinergic immunotoxin
Ferreira, G, et al
Neuroscience, 104:419-39 (2001)  2001

11377845 11377845
Antibody for human p75 LNTR identifies cholinergic basal forebrain of non-primate species
Tremere, L A, et al
Neuroreport, 11:2177-83 (2000)  2000

10923666 10923666
Characterization of nerve growth factor receptor in neural crest tumors using monoclonal antibodies
Ross, A H, et al
Proc Natl Acad Sci USA, 81:6681-5 (1984)  1984

6093111 6093111

Data Sheet

Title
MOUSE ANTI-NERVE GROWTH FACTOR-RECEPTOR, p75 ALEXA FLUOR® 488 CONJUGATED MONOCLONAL ANTIBODY

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies