Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MAB1592-C Anti-Neurofilament NF-H Antibody, phosphorylated Antibody, clone NP1

Anti-Neurofilament NF-H, phosphorylated, clone NP1, Cat. No. MAB1592-C, is a highly specific mouse monoclonal antibody that targets Neurofilament heavy polypeptide and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: NEFH, KIAA0845, NFH UniProt Number: P12036
View Products on Sigmaaldrich.com
MAB1592-C
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, M, CaELISA, IH(P), WBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB1592-C
DescriptionAnti-Neurofilament NF-H Antibody, phosphorylated Antibody, clone NP1
Alternate Names
  • Neurofilament heavy polypeptide
  • NF-H
  • 200 kDa neurofilament protein
  • Neurofilament triplet H protein
Background InformationNeurofilament heavy polypeptide (UniProt: P12036; also known as NF-H, 200 kDa neurofilament protein, Neurofilament triplet H protein) is encoded by the NEFH (also known as KIAA0845, NFH) gene (Gene ID: 4744) in human. Neurofilaments (~ 10 nm in diameter) are intermediate filaments that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. They are built from three intertwined protofibrils, which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Antibodies to NF-H are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-H antibodies are helpful in the identification of neurofilament accumulations seen in many neurological diseases, such as Amyotrophic lateral sclerosis (ALS) and Alzheimer's disease. The purified immunogen used to generate this antibody was the very heavily phosphorylated form of NF-H and this antibody binds only this heavily phosphorylated form. Similar, but far fewer phosphorylation sites are also found in NF-M. Hence, this antibody displays some reactivity with NF-M. Enzymatic phosphorylation of porcine and bovine neurofilaments is shown to result in loss of binding.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in PBS with 5 mM sodium azide and 50% glycerol.
Quality LevelMQ100
Applications
ApplicationAnti-Neurofilament NF-H, phosphorylated, clone NP1, Cat. No. MAB1592-C, is a highly specific mouse monoclonal antibody that targets Neurofilament heavy polypeptide and has been tested for use in ELISA, Immunohistochemistry (Paraffin), and Western Blotting.
Key Applications
  • ELISA
  • Immunohistochemistry (Paraffin)
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Neurofilament NF-H in human ovary tissue.

Immunohistochemistry Analysis: A representative lot detected Neurofilament NF-H in Immunohistochemistry applications (Benson, D.L., et. al. (1996). J Neurocytol. 25(3):181-96; Boylan, K., et. al. (2009). J Neurochem. 111(5):1182-91).

Western Blotting Analysis: A representative lot detected Neurofilament NF-H in Western Blotting applications (Benson, D.L., et. al. (1996). J Neurocytol. 25(3):181-96; Boylan, K., et. al. (2009). J Neurochem. 111(5):1182-91).

ELISA Analysis: A representative lot detected Neurofilament NF-H in ELISA applications (Boylan, K., et. al. (2009). J Neurochem. 111(5):1182-91; Toedebusch, C.M., et. al. (2017). J Vet Intem Med. 31(2):513-520; Mashita, T., et. al. (2015). J Vet Med Sci. 77(4):433-8).
Biological Information
ImmunogenNative Neurofilament H purified from pig spinal cord.
CloneNP1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone NP1 detects heavily phosphorylated form of Neurofilament H. Displays weaker reactivity with phosphorylated Neurfilament -M.
IsotypeIgG1κ
Species Reactivity
  • Rat
  • Human
  • Mouse
  • Canine
Species Reactivity NoteCanine, Rat, Human, Mouse. Predicted to react with Chicken, Bovine, Porcine based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NEFH
  • KIAA0845
  • NFH
Purification MethodProtein G purified
UniProt Number
Molecular Weight112.48 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry in human cerebral cortex tissue.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Neurofilament NF-H in human cerebral cortex tissue.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB1592-C 04054839396755

Documentation

Anti-Neurofilament NF-H Antibody, phosphorylated Antibody, clone NP1 Certificates of Analysis

TitleLot Number
Anti-Neurofilament NF-H, phosphorylated, clone NP1 - 3539777 3539777
Anti-Neurofilament NF-H, phosphorylated, clone NP1 - 3729334 3729334
Anti-Neurofilament NF-H, phosphorylated, clone NP1 - 4028604 4028604
Anti-Neurofilament NF-H, phosphorylated, clone NP1 - 4080524 4080524
Anti-Neurofilament NF-H, phosphorylated, clone NP1 - 4166524 4166524
Anti-Neurofilament NF-H, phosphorylated, clone NP1 Monoclonal Antibody Q2959730