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ABN2252-100UG Anti-Neuroligin-4

Anti-Neuroligin-4, Cat. No. ABN2252, is a rabbit polyclonal antibody that detects Neuroligin-4 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

ABN2252-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABN2252-100UG
DescriptionAnti-Neuroligin-4
Alternate Names
  • Neuroligin Y
Background InformationNeuroligin-4 (UniProt: Q8NFZ3; also known as Neuroligin Y) is encoded by the NLGN4Y (also known as KIAA0951) gene (Gene ID: 22829) in human. Neuroligins are postsynaptic cell adhesion molecules that have crucial roles in synapse maturation and function. In humans, NLGN4X is located on the X chromosome and the NLGN4Y is localized on the Y chromosome and together they form an X-Y pair. They share about 97% amino acid identity; however, they are differentially regulated. Although several mutations in NLGN4X have been linked to autism, only one mutation has been identified in NLGN4Y gene. Despite their high degree of sequence identity, NLGN4Y exhibits severe deficit in trafficking to cell surface and related synaptic effects. This has been attributed to serine 93 in NLGN4Y. A substitution of serine to proline at residue 93 is shown to rescue it and increase its surface trafficking. Neuroligin-4 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-43), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 44-676), a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). Its expression is observed in fetal and adult brain, prostate, and testis. It serves as a neuronal cell surface protein that is involved in cell-cell interactions. (Ref.: Nguyen, TA., et al. (2020). Neuron 106(5); 759-768; Marro, SG., et al. (2019). Neuron. 103(4); 617-626).
References
Product Information
FormatPurified
PresentationPurified rabbit polyclonal antibody in buffer containing PBS, pH 7.4, without azide.
Quality LevelMQ200
Applications
ApplicationAnti-Neuroligin-4, Cat. No. ABN2252, is a rabbit polyclonal antibody that detects Neuroligin-4 and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
Application NotesTested applications

Immunofluorescence Analysis: A representative lot detected Neuroligin-4 in Immunofluorescence applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Western Blotting Analysis: A representative lot detected Neuroligin-4 in Western Blotting applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Neuroligin-4 in Immunoprecipitation applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human Neuroligin-4.
EpitopeC-terminal cytoplasmic domain
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Neuroligin-4. It targets an epitope within 16 amino acids from the C-terminal, cytoplasmic domain.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Monkey based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • NLGN4Y
Purification MethodAffinity Purified
UniProt Number
Molecular Weight~110 kDa observed; 92.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells overexpressing Human Neuroligin-4.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neuroligin-4 in lysate from HEK293T cells overexpressing Human Neuroligin-4.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN2252-100UG 04065270172440