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AB15332 Anti-Nicotinic Acetylcholine Receptor α7 Antibody

Anti-Nicotinic Acetylcholine Receptor 7, Cat. No. AB15332, is a rabbit polyclonal antibody that detects Nicotinic Acetylcholine Receptor 7 and is tested for use in Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: CHRNA7, NACHRA7 UniProt Number: P36544
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AB15332
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, MWBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB15332
ReplacesAB5637
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Nicotinic Acetylcholine Receptor α7 Antibody
Background InformationNeuronal acetylcholine receptor subunit alpha-7 (UniProt: P36544; also known as -Nicotinic Receptor Alpha 7, Nicotinic AChR alpha7) is encoded by the CHRNA7 (also known as NACHRA7) gene (Gene ID: 1139) in human. Nicotinic acetylcholine receptors are cation-selective, ligand-gated ion channels that mediate fast neurotransmission. They are distributed at the post-synaptic membrane of muscle cells. A similar neuronal-type ACh receptor is also found in the central nervous system and is involved in a wide range of physiological and pathological functions. They are assembled either as homo-pentamers of 7, 8, and 9 or hetero-pentamers of 2 6 in combination with β2 β4 or 9 with 10 subunits. They regulate the flow of sodium, potassium, and calcium ions across the cell membrane. The binding of ligands to these receptors triggers a tertiary conformational transition of neuronal AChRs among functionally distinct resting, open, and desensitized states. Nicotinic AChR 7 is a multi-pass, homopentameric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-230), four transmembrane domains (aa 231-255; 262-280; 296-317; and 470-490), and one cytoplasmic domain (aa 318-469). (Ref.: Ho, TN., et al. (2020). Front. Neurosci. 14; 609005; Unwin, N. (2013). Q. Rev. Biophys. 46(4); 283-322).
References
Product Information
FormatAffinity Purified
HS Code3002 15 90
PresentationPurified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Nicotinic Acetylcholine Receptor 7, Cat. No. AB15332, is a rabbit polyclonal antibody that detects Nicotinic Acetylcholine Receptor 7 and is tested for use in Western Blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal half of human Nicotinic Receptor 7.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityThis rabbit polyclonal antibody specifically detects Nicotinic Receptor 7. It targets an epitope with 28 amino acids from the C-terminal half.
Species Reactivity
  • Human
  • Rat
  • Mouse
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene.
Gene Symbol
  • CHRNA7
  • NACHRA7
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P36544 # After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
SIZE: 502 amino acids; 56449 Da
SUBUNIT: Homopentamer. Interacts with RIC3; which is required for proper folding and assembly.
SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P36544 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
Molecular WeightTarget molecular weight 52 kDa observed. 56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Nicotinic Acetylcholine Receptor 7 in Rat brain tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB15332 04053252329036

Documentation

Anti-Nicotinic Acetylcholine Receptor α7 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Nicotinic Acetylcholine Receptor α7 Antibody Certificates of Analysis

TitleLot Number
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2433491 2433491
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 - 3211114 3211114
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 - 3933300 3933300
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2630253 2630253
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2723587 2723587
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2762299 2762299
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2794884 2794884
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2884554 2884554
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2040904 2040904
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2066012 2066012

Data Sheet

Title
RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7