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06-284-AF488 Anti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate

Anti-Nitrotyrosine AlexaFluor® 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling
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06-284-AF488
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HICCRbAlexaFluor®488Polyclonal Antibody
Description
Catalogue Number06-284-AF488
DescriptionAnti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate
Alternate Names
  • Nitrotyrosine AlexaFluor® 488 Conjugate
Background InformationNitrotyrosine is produced by the action of reactive nitrogen species, such as peroxinitrite and nitrogen dioxide on tyrosine residues in proteins. Nitrotyrosine is an indicator of cell damage, inflammation, and nitric oxide production. Both free tyrosine and protein bound tyrosine residues are prone to nitration. In several pathological conditions, oxidative stress is increased resulting in the formation of super oxide and nitric oxide that react to form peroxynitrite (ONOO-), which is a highly reactive molecule. It causes oxidation of proteins and lipids and DNA damage, which results in loss of protein and lipid function and can increase the probability of gene mutations. Higher incidence of protein nitration has been reported in diabetes, atherosclerotic plaques, and rheumatoid arthritis, among others. Nitrotyrosine can be measured by amino-acid analysis of protein hydrolysates and detected cells and tissues by immunocytochemical techniques using antibodies developed against the nitrotyrosine.
References
Product Information
FormatAlexaFluor®488
PresentationPurified Rabbit IgG in buffer containing 0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl,and 0.05% sodium azide, 10mg/ml BSA.
Quality LevelMQ100
Applications
ApplicationAnti-Nitrotyrosine AlexaFluor® 488 Conjugate, Cat. No. 06-284-AF488, is a rabbit polyclonal antibody that detects tyrosine nitrated proteins and has been tested for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antibody (Cat. No. 06-284) is shown to be suitable also for Western blotting, immunocytochemistry, immunohistochemistry, and immunoprecipitation.
Biological Information
ImmunogenNitrated Keyhole Limpet hemocyanain (KLH).
EpitopeNitrated Tyrosine
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects tyrosine nitrated proteins.
IsotypeIgG
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with all species.
Antibody TypePolyclonal Antibody
Purification MethodAffinity Purfied
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in A431 cells treated with peroxytnitrite.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Nitrotyrosine in A431 cells treated with 5 mM peroxytnitrite.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
06-284-AF488 04055977298840

Documentation

Anti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Nitrotyrosine Antibody AlexaFluor® 488 Conjugate Certificates of Analysis

TitleLot Number
Anti-Nitrotyrosine AlexaFluor® 488 -Q2610558 Q2610558
Anti-Nitrotyrosine AlexaFluor® 488 Conjugate 2943137
Anti-Nitrotyrosine AlexaFluor® 488 Conjugate 2902697
Anti-Nitrotyrosine AlexaFluor® 488 Conjugate - 3314246 3314246
Anti-Nitrotyrosine AlexaFluor® 488 Conjugate - 3524736 3524736
Anti-Nitrotyrosine AlexaFluor® 488 Conjugate - 4096905 4096905
Anti-Nitrotyrosine AlexaFluor® 488 Conjugate -2834502 2834502
Anti-Nitrotyrosine AlexaFluor® 488 Conjugate Polyclonal Antibody 3091232

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New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

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Post-Translational Modifications