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MABS1164 Anti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1

This Anti-Nucleobindin-2 Antibody, clone 3B3.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Nestafin-1.

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Research Category: Signaling Research Sub-Category: Metabolic Hormones & Receptors Gene Symbol: Nucb2, Nefa UniProt Number: Q9JI85
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MABS1164
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RWB, IH(P)MPurifiedMonoclonal Antibody
Description
Catalogue NumberMABS1164
DescriptionAnti-Nucleobindin-2/Nestafin-1 Antibody, clone 3B3.1
Alternate Names
  • Nucleobindin-2
  • Nestafin-1
  • DNA-binding protein NEFA
  • Prepronesfatin
  • Pronesfatin
Background InformationNucleobindin-2 (UniProt Q9JI85; also known as DNA-binding protein NEFA, Prepronesfatin) is encoded by the Nucb2 (also known as Nefa) gene (Gene ID 59295) in rat species. Nucleobindin-2 is initially translated as a 420-a.a. precursor protein. Cleavage of signal peptide (aa1-24) yields the 396-a.a. mature nucleobindin-2 with very high sequence homology among human, mouse, and rat species (more than 85%). Post-translational processing by prohormone convertases (PC 1/3 and PC 2) cleaves nucleobindin-2 into nesfatin-1, nesfatin-2, and nesfatin-3 (aa1–82, aa85–163, and aa166–396, respectively, numbering based on mature sequence). Nesfatin-1 is a well recognized circulating anorexigenic peptide, whose production increases post-prandially. Nucleobindin-2 contains two calcium-binding EF-hand domains separated by an acidic amino acid-rich region, and a leucine zipper. Nucleobindin-2 is known to be expressed in key hypothalamic nuclei and plays a role in energy homeostasis. Nucleobindin-2 is also expressed in various human peripheral tissues, including the stomach, pancreas, reproductive organs, and adipose tissues, suggesting roles in various metabolic functions. Nucleobindin-2 expression level is reported to be elevated in clear cell renal cell carcinoma (ccRCC), breast cancer, and prostate cancer (PCa) tissues.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Nucleobindin-2 Antibody, clone 3B3.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Nestafin-1.
Key Applications
  • Western Blotting
  • Immunohistochemistry (Paraffin)
Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected nucleobindin-2/nestafin-1 immunoreactivity in human colon cancer tissue, as well as in normal testis, tonsil, and pancreas tissue sections.
Biological Information
ImmunogenGST-tagged recombinant rat Nestafin-1.
EpitopeNesfatin-1
Clone3B3.1
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityClone 3B3.1 reacts with pro-nucleobindin-2, mature nucleobindin-2, and nesfatin-1, but not nesfatin-2 or nesfatin-3.
IsotypeIgG1κ
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteMouse, Human, Rat.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Nucb2
  • Nefa
Purification MethodProtein G purified
UniProt Number
Molecular Weight~50 kDa observed. 47.33/50.20 kDa (human mature/pro-nucleobindin-2), 47.42/50.30 kDa (mouse mature/pro-nucleobindin-2), 47.25/50.09 kDa (rat mature/pro-nucleobindin-2), 9.552/9.612/9.583 kDa (human/mouse/rat nesfatin-1) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected nucleobindin-2 in 10 µg of mouse liver tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS1164 04055977353556