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MABE1043 Anti-Nup62 Antibody, clone 2A11

Anti-Nup62 Antibody, clone 2A11 is an antibody against Nup62 for use in Western Blotting and Immunocytochemistry.

Anti-Nup62 Antibody, clone 2A11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: NUP62 UniProt Number: P37198
MABE1043
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, ICCRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE1043
DescriptionAnti-Nup62 Antibody, clone 2A11
Alternate Names
  • Nuclear pore glycoprotein p62
  • Nup62
  • 62 kDa nucleoporin
  • Nucleoporin Nup62
Background InformationNuclear pore glycoprotein p62 (UniProt P37198; also known as 62 kDa nucleoporin, Nucleoporin Nup62) is encoded by the NUP62 (Also known as IBSN, SNDI) gene in human (Gene ID 23636). The nuclear pore complex (NPC) acts as a passageway for nucleocytoplasmic transport. Soluble cargo-protein complexes navigate through the pore by binding to phenylalanine-glycine (FG)-repeat proteins attached to the channel walls. The Nup62 complex is located in the center of the NPC and contains the FG-repeat proteins Nup62, Nup54, and Nup58 associated with each other via conserved coiled-coil segments in a 1:1:1 stiochiometric ratio. The nuclear envelope breaks down during prometaphase. The disassembled nucleoporin subcomplexes act as critical regulators of various mitotic events to ensure proper chromosome segregation. Specifically, Nup62 is reported to localize to centrosomes/spindle poles, where it associates with the centrosomal proteins gamma-tubulin and hSAS-6. Nup62 is involved in the recruitment of critical proteins to the centrosome and its depletion results in mislocalization of several centrosomal components.
References
Product Information
FormatPurified
PresentationPurified rat monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ300
Applications
ApplicationAnti-Nup62 Antibody, clone 2A11 is an antibody against Nup62 for use in Western Blotting and Immunocytochemistry.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Nup62 in HeLa cell nuclear membrane fraction (Fukuhara, T., et al. (2006). Hybridoma. 25(2):51-59).
Immunocytochemistry Analysis: A representative lot detected Nup62 in HeLa cells (Fukuhara, T., et al. (2006). Hybridoma. 25(2):51-59).
Immunocytochemistry Analysis: A representative lot detected Nup62 in HeLa S3 cells (Maeshima, K., et al. (2006). J. of Cell Science. 119:4442-4451).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to aa1-179 of human Nup62.
EpitopeWithin aa1-179.
Clone2A11
ConcentrationPlease refer to lot specific datasheet.
HostRat
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • NUP62
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~62 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa and Raji cell lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected Nup62 in 10 µg of HeLa and Raji cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE1043 04055977169850

Documentation

Anti-Nup62 Antibody, clone 2A11 SDS

Title

Safety Data Sheet (SDS) 

Anti-Nup62 Antibody, clone 2A11 Certificates of Analysis

TitleLot Number
Anti-Nup62, clone 2A11 - 3257008 3257008
Anti-Nup62, clone 2A11 - 3536037 3536037
Anti-Nup62, clone 2A11 - 3885983 3885983
Anti-Nup62, clone 2A11 -Q2569119 Q2569119
Anti-Nup62, clone 2A11 Monoclonal Antibody 3083531

Technical Info

Title
Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
White Paper: Further considerations of antibody validation and usage.