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ABE2895 Anti-ORF0

Anti-ORF0 Antibody, Cat. No. ABE2895, is a highly specific rabbit polyclonal antibody that targets ORF0 and has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

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ABE2895
100 µL  
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Overview

Replacement Information
Description
Catalogue NumberABE2895
DescriptionAnti-ORF0
Alternate Names
  • ORF0 protein
  • Open Reading Frame 0
Background InformationOpen Reading Frame 0 (ORF0) is a primate-specific open reading frame in LINE-1 retrotransposons. Over 3,000 potential ORF0 loci are reported to be present in human and chimpanzee genomes. ORF0 is a predominantly nuclear protein that enhances LINE-1 mobility and contributes to retrotransposon-mediated diversity. It is a highly positively charged protein that may act by binding to nucleic acids. It displays two splice donor (SD) sites at nucleotides 106 and 191 (amino acids 35 and 64) that can act in concert with splice acceptors (SAs) in downstream genomic sequences to generate fusion proteins. ORF0 transcripts are reported to exist in induced pluripotent stem cells (iPSc) derived from primates. (Ref.: Denli, AM et al (2015). Cell 163(3): 583 593).
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-ORF0 Antibody, Cat. No. ABE2895, is a highly specific rabbit polyclonal antibody that targets ORF0 and has been tested in Immunocytochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunocytochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).

Immunoprecipitation Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).

Immunocytochemistry Analysis: A representative lot detected ORF0 in HEK293T cells transfected with ORF0 (Denli, A.M., et. al. (2015). Cell. 163(3):583-93).
Biological Information
ImmunogenKLH-conjugated linear peptides corresponding to ORF0 amino acid residues 20-34, 33-49, and 50-65.
Epitopeunknown
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody recognizes primate-specific open reading frame 0 (ORF0).
IsotypeIgG
Species Reactivity
  • Primate
Species Reactivity NotePrimate. Predicted to react with Chimpanzee and Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Gene Symbol
  • ORF0
Purification MethodUnpurified
UniProt Number
Molecular Weight~16 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in extracts from HEK293T cells transfected with ORF0 expression plasmid.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected ORF0 in lysate from HEK293T cells transfected with ORF0 expression plasmid.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABE2895 04054839337130

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