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MABE2036-25UG Anti-PADI2/PAD2 Antibody, clone DN2

Anti-PADI2/PAD2, clone DN2, Cat. No. MABE2036, is a mouse monoclonal antibody that detects Protein-arginine deiminase type-2 (PADI2) and is tested in ELISA, Immunohistochemistry, Multiplexing, and Western Blotting.

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25 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABE2036-25UG
DescriptionAnti-PADI2/PAD2 Antibody, clone DN2
Alternate Names
  • Peptidylarginine deiminase 2
  • PAD2
Background InformationProtein-arginine deiminase (UniProt: A0A5F9DB16; also known as PADI2, PAD2) is encoded by the PADI2 gene (Gene ID: 100357368) in rabbit. Protein-arginine deiminases (PAD) catalyze the hydrolysis of peptidyl-arginine to peptidyl-citrulline and alter protein function. Five isoforms of PAD have been described in mammals (PAD1, 2, 3, 4, and 6) that are homologous in their catalytic site but are expressed differently in different cells and tissues and have different physiological roles. PAD2, one of the most abundant PADs has three domains: N-terminal domain (aa 1-114), a central domain (aa 116-274), and a C-terminal domain (aa 285-657). High calcium concentration is reported to be a prerequisite for PAD2 activation. A redox potential maintains its catalytic site in a reduced state. Overexpression of PAD2 has been associated with inflammatory diseases, such as rheumatoid arthritis and neurodegenerative diseases including multiple sclerosis. Higher levels of PAD2 are observed in transformed cell model of breast cancer progression with increasing tumor malignancy and in a murine models of xenograft tumors. Clone DN2 binds to an epitope in the N-terminal region and is ideally suited for quantitative ELISA. (Ref.: Wang, Y., et al. (2021). Int. J. Cancer. 148(2); 267-276; Vejlstrup, A., et al. (2019). J. Inflamm. Res. 12; 137 144; Damgaard, D., et al. (2016). Rheumatology 55(5); 918-927; Damgaard, D., et al. (2014). J. Immunol. Methods. 405; 15-22).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-PADI2/PAD2, clone DN2, Cat. No. MABE2036, is a mouse monoclonal antibody that detects Protein-arginine deiminase type-2 (PADI2) and is tested in ELISA, Immunohistochemistry, Multiplexing, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunohistochemistry
  • Multiplexing
  • ELISA
Application NotesTested Applications

Immunohistochemistry Applications: A representative lot detected PADI2/PAD2 in Immunohistochemistry applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22).

Western Blotting Analysis: A representative lot detected PADI2/PAD2 in Western Blotting applications (Damgaard, D., et al. (2014). J Immunol Methods. 405:15-22; Bawadekar, M., et al. (2017). J Autoimmun. 80:39-47).

ELISA Analysis: A representative lot detected PADI2/PAD2 in ELISA applications (Damgaard, D., et al. (2014). J Immunol Methods.;405:15-22; Damgaard, D., et al. (2016). Rheumatology (Oxford). 55(5):918-27).

Multiplexing: A representative lot detected PADI2/PAD2 in Multiplexing applications (Vejlstrup, A., et al. (2019). J Inflamm Res. 29:12:137-144).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenProtein-arginine deiminase (PADI2/PAD2) from rabbit skeletal muscle.
EpitopeN-terminal
CloneDN2
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone DN2 is a mouse monoclonal antibody that detects Protein-arginine deiminase 2 (PADI2/PAD2). It targets an epitope within the N-terminal region.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rabbit based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PADI2
Purification MethodProtein G purified
UniProt Number
Molecular Weight~80 kDa observed; 74.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with full-length, recombinant, Human PADI2/PAD2.

Western Blotting Analysis: A 1:500 dilution of this antibody detected full-length, recombinant, Human PADI2/PAD2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE2036-25UG 04065271058972