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PC474 Anti-PCNA (247-261) (Ab-5) Rabbit pAb

This Anti-PCNA (247-261) (Ab-5) Rabbit pAb is validated for use in Immunoprecipitation for the detection of PCNA (247-261) (Ab-5).

Anti-PCNA (247-261) (Ab-5) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Proliferating Cell Nuclear Antigen

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PC474
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Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
HRbPolyclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
PC474-50UL Plastic ampoule 50 ul
Description
OverviewRecognizes the ~37 kDa PCNA protein in MCF-7 cells.
Catalogue NumberPC474
Brand Family Calbiochem®
SynonymsAnti-Proliferating Cell Nuclear Antigen
Application Data
Detection of human PCNA by immunoprecipitation. Sample: Whole cell lysate from MCF7 cells. Antibodyfor IP: Anti-PCNA (Ab-1) Mouse mAb (PC10) (Cat. No. NA03) (1 µg/ml). Anti-PCNA (247-261) (Ab-5) Rabbit pAb (Cat. No. PC474) (1:1000). Detection: chemiluminescence. A nonspecific band appears at ~45 kDa in the sample lysate and in all the controls.
References
ReferencesMiyachi, K., et al. 1990. J. Immunol. 121, 2228.
Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
Bravo, R., et al. 1987. Nature (London) 326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
Product Information
FormLiquid
FormulationUndiluted serum.
Positive controlMCF7 cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (1:250-1:1000)
Application CommentsWill immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 247-261 of human PCNA
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
PC474-50UL 04055977208337

Documentation

Anti-PCNA (247-261) (Ab-5) Rabbit pAb SDS

Title

Safety Data Sheet (SDS) 

Anti-PCNA (247-261) (Ab-5) Rabbit pAb Certificates of Analysis

TitleLot Number
PC474

References

Reference overview
Miyachi, K., et al. 1990. J. Immunol. 121, 2228.
Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
Bravo, R., et al. 1987. Nature (London) 326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision10-March-2008 JSW
SynonymsAnti-Proliferating Cell Nuclear Antigen
ApplicationImmunoprecipitation (1:250-1:1000)
Application Data
Detection of human PCNA by immunoprecipitation. Sample: Whole cell lysate from MCF7 cells. Antibodyfor IP: Anti-PCNA (Ab-1) Mouse mAb (PC10) (Cat. No. NA03) (1 µg/ml). Anti-PCNA (247-261) (Ab-5) Rabbit pAb (Cat. No. PC474) (1:1000). Detection: chemiluminescence. A nonspecific band appears at ~45 kDa in the sample lysate and in all the controls.
DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~36-37 kDa PCNA protein.
BackgroundThe proliferating cell nuclear antigen (PCNA) is a ~36-37 kDa protein also known as cyclin. The protein has been identified as a polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase PCNA has a very granular distribution and is absent from the nucleloli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cell fixed with aldehydes the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have autoantibodies against PCNA.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide corresponding to amino acids 247-261 of human PCNA
IsotypeIgG
Specieshuman
Positive controlMCF7 cells
FormLiquid
FormulationUndiluted serum.
Preservative≤0.1% sodium azide
CommentsWill immunoprecipitate PCNA when complexed with other proteins. Antibody should be titrated for optimal results in individual systems.
Storage Avoid freeze/thaw
-20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMiyachi, K., et al. 1990. J. Immunol. 121, 2228.
Waseem, N.H. and Lane, D.P. 1990. Science 96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 3189.
Bravo, R., et al. 1987. Nature (London) 326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.