Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

NA03 Anti-PCNA (Ab-1) Mouse mAb (PC10)

This Anti-PCNA (Ab-1) Mouse mAb (PC10) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation Paraffin Sections for the detection of PCNA (Ab-1).

Anti-PCNA (Ab-1) Mouse mAb (PC10) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Proliferating Cell Nuclear Antigen

View Products on Sigmaaldrich.com
NA03
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityHostAntibody Type
H, M, R, Yeast, ZebrafishMMonoclonal Antibody

Products

Catalog NumberPackaging Qty/Pack
NA03-200UG Plastic ampoule 200 μg
Description
OverviewRecognizes the ~37 kDa PCNA protein in HeLa cells as well as in tonsil or normal colon tissue.
Catalogue NumberNA03
Brand Family Calbiochem®
SynonymsAnti-Proliferating Cell Nuclear Antigen
References
ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
Bravo, R., et al. 1987. Nature (London) 326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
Product Information
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Immunofluorescence
Not Immunoprecipitation
Paraffin Sections
Application NotesImmunoblotting (2.5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
Application CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogenrecombinant PCNA
EpitopeWithin amino acids 112-121
ClonePC10
HostMouse
IsotypeIgG2a
Species Reactivity
  • Human
  • Mouse
  • Rat
  • Yeast
  • Zebrafish
Antibody TypeMonoclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
NA03-200UG 04055977227642

Documentation

Anti-PCNA (Ab-1) Mouse mAb (PC10) SDS

Title

Safety Data Sheet (SDS) 

Anti-PCNA (Ab-1) Mouse mAb (PC10) Certificates of Analysis

TitleLot Number
NA03

References

Reference overview
Waseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
Bravo, R., et al. 1987. Nature (London) 326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
Miyachi, K., et al. 1987. J. Immunol. 121, 2228.

Citations

Title
Goessling, et al. 2008. Developmental Biology 320, 161.
Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
SynonymsAnti-Proliferating Cell Nuclear Antigen
ApplicationImmunoblotting (2.5 µg/ml)
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (not recommended with unconjugated antibody, see comments)
Paraffin Sections (2.5 µg/ml, no pre-treatment required, see comments)
DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0-Ag14 mouse myeloma cells. Recognizes the ~37 kDa PCNA protein.
BackgroundThe proliferating cell nuclear antigen (PCNA) is a 37 kDa molecular weight protein also known as cyclin. The protein has also been identified as the polymerase δ accessory protein and is detected in a cell cycle dependent manner. In early S phase, PCNA has a very granular distribution and is absent from the nucleoli. At late S phase, PCNA is prominent in the nucleoli. In cells fixed with organic solvents, the PCNA is seen to be strongly associated in the nuclear regions where DNA synthesis is occurring, whereas in cells fixed with aldehydes, the staining is more diffuse but intense and occurs throughout the cell cycle. This is due to the presence of two basic forms of the PCNA protein: a soluble form which is sensitive to organic fixation and not involved in replication and a second form which is insoluble and associated with on-going DNA synthesis. PCNA is a very conserved protein present not only in mammals but also in plant cells. Patients suffering from SLE have been shown to have auto antibodies against PCNA.
HostMouse
Immunogenrecombinant PCNA
EpitopeWithin amino acids 112-121
ClonePC10
IsotypeIgG2a
Specieshuman, mouse, rat, yeast, zebrafish
Positive controlHeLa cells, HL-60 cells, tonsil tissue, or normal colon tissue
FormLiquid
FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsStaining shows an intense nuclear pattern. Not recommended for immunoprecipitation because in some samples a ~37 kDa protein will be precipitated that is not PCNA. Generally, no pre-treatment is needed to stain paraffin sections, but staining may be enhanced in some samples by pretreating with 4 N HCl for 10 min at 37°C followed by 3 washes in water. Antibody should be titrated for optimal results in individual systems.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesWaseem, N.H. and Lane, D.P. 1990. J. Cell. Sci. 96, 121.
Suzuka, I., et al. 1989. Proc. Natl. Acad. Sci. USA 86 3189.
Bravo, R., et al. 1987. Nature (London) 326, 515.
Bravo, R. and MacDonald-Bravo, H. 1987. J. Cell. Biol. 105, 1549.
Miyachi, K., et al. 1987. J. Immunol. 121, 2228.
CitationGoessling, et al. 2008. Developmental Biology 320, 161.