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ABF133 Anti-PD-L1 Antibody/CD274

Anti-PD-L1 Antibody/CD274 is an antibody against PD-L1/CD274 for use in western blotting & flow cytometry.

Anti-PD-L1 Antibody/CD274 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunological Signaling Gene Symbol: CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1 UniProt Number: Q9NZQ7
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ABF133
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWB, FCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABF133
DescriptionAnti-PD-L1 Antibody/CD274
Alternate Names
  • Programmed cell death 1 ligand 1
  • PD-L1
  • PDCD1 ligand 1
  • Programmed death ligand 1
  • B7 homolog 1
  • B7-H1
  • CD274
Background InformationProgrammed cell death 1 ligand 1 (PD-L1/CD274) is a B7 related protein belonging to the BTN/MOG family. PDL-1/CD274 is essential for T-cell proliferation and production of IL10 and IFNG. PD-L1/CD274 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PD-L1/CD274 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PD-L1/CD274 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PD-L1 Antibody/CD274 is an antibody against PD-L1/CD274 for use in western blotting & flow cytometry.
Key Applications
  • Western Blotting
  • Flow Cytometry
Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected PD-L1/CD274 in 10 µg of K562 cell lysate.

Flow Cytometry Analysis: 0.25 µg from a representative lot detected PD-L1/CD274 in 1X10E6 K562 cells.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to the extracellular domain of human PD-L1/CD274.
EpitopeThis antibody recognizes the Ig-like C2-type domain in the extracellular domain of PD-L1/CD274.
Concentration1 mg/mL
HostRabbit
SpecificityThis antibody should recognize isoforms 1 and 2 based upon immunogen design.
Species Reactivity
  • Human
Species Reactivity NoteHuman. Predicted to react with Rhesus Macaque based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • CD274
  • B7H1
  • PDCD1L1
  • PDCD1LG1
  • PDL1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~55 kDa and ~33 kDa observed. Uniprot describes three isoforms at 33 kDa, 20 kDa and 21 kDa. This antibody contains 4 known glyscosylation sytes. An unglycosylated and glycosylated/membrane form may be observed at 34 kDa and 55 kDa, respectively.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PD-L1/CD274 in 10 µg of Jurkat cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABF133 04053252971297