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MABC1131-100UG Anti-PD-L1 B7-H1 Antibody, clone 10B5

Anti-PD-L1 B7-H1, clone 10B5, Cat. No. MABC1131, is an Armenian hamster monoclonal antibody that detects Programmed cell death 1 ligand 1 (PD-L1) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Inhibition Function assay.

Anti-PD-L1 B7-H1 Antibody, clone 10B5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1 UniProt Number: Q9EP73
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MABC1131-100UG
100 μg  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MFC, IH(P), INHIBAHmPurifiedMonoclonal Antibody
Description
Catalogue NumberMABC1131-100UG
DescriptionAnti-PD-L1 B7-H1 Antibody, clone 10B5
Alternate Names
  • Programmed cell death 1 ligand 1
  • PDCD1 ligand 1
  • Programmed death ligand 1
  • B7 homolog 1
  • B7-H1
  • CD274
Background InformationProgrammed cell death 1 ligand 1 (UniProt: Q9EP73; also known as PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274) is encoded by the Cd274 (also known as B7h1, Pdcd1l1, Pdcd1lg1, Pdl1) gene (Gene ID: 50533) in murine species. PD-L1 is a single-pass type I membrane protein that is highly expressed in the heart, thymus, skeletal muscle, and lung. Its expression is also reported on activated dendritic cells, B-cells, and macrophages. Numerous tumor cell lines of lymphoid origin also express PD-L1. PD-L1 expression can be up-regulated by interferon-gamma treatment in monocytes, B cells, and macrophages. PD-1 and its ligands, PD-L1 and PD-L2 play a key role in the maintenance of peripheral tolerance, a process by which the quiescence of autoreactive mature T cells is maintained. However, tumors and pathogens that cause chronic infections can exploit this pathway to escape T-cell mediated tumor-specific and pathogen-specific immunity. The effector functions of T-cells expressing PD-1 can be downregulated by PD-L1 or PD-L2 expressed by the tumor cells. PD-L1 is initially produced with signal peptide (aa. 1-18) sequence, the removal of which yields the mature protein with a large extracellular (aa. 19-239) domain, a transmembrane domain (aa 240-260), and a cytoplasmic domain (aa 261-290).
References
Product Information
FormatPurified
PresentationPurified armenian hamster monoclonal antibody IgG2-IgG3 in PBS without azide.
Applications
ApplicationAnti-PD-L1 B7-H1, clone 10B5, Cat. No. MABC1131, is an Armenian hamster monoclonal antibody that detects Programmed cell death 1 ligand 1 (PD-L1) and has been tested for use in Flow Cytometry, Immunohistochemistry (Paraffin), and Inhibition Function assay.
Key Applications
  • Flow Cytometry
  • Immunohistochemistry (Paraffin)
  • Inhibits Activity/Function
Application NotesInhibits Activity/Function Analysis: A representative lot blocked the activity of PD-L1 B7-H1 and inhibited apoptosis of activated alloreactive 2C T cells. (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Goldberg, M.V., et. al. (2007). Blood. 110(1):186-92; Joshi, T., et. al. (2009). PLoS Pathog. 5(5):e1000431; Liu, X., et. al. (2016). Sci Rep. 6:36722).



Flow Cytometry Analysis: A representative lot detected PD-L1 B7-H1 in Flow Cytometry applications (Hirano, F., et. al. (2005). Cancer Res. 65(3):1089-96; Dong, H., et. al. (2004). Immunity. 20(3):327-36).
Biological Information
ImmunogenRecombinant fragment corresponding to the extracellular domain of Mouse Programmed cell death 1 ligand 1.
Epitopeextracellular domain
Clone10B5
ConcentrationPlease refer to lot specific datasheet.
HostArmenian Hamster
SpecificityThis Armenian hamster monoclonal antibody detects Programmed cell death 1 ligand 1 (PD-L1) in murine species.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Cd274
  • B7h1
  • Pdcd1l1
  • Pdcd1lg1
  • Pdl1
Purification MethodProtein G purified
UniProt Number
Molecular Weight32.78 kDa calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in mouse heart tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:25 dilution of this antibody detected PD-L1 B7-H1 in mouse heart tissue sections.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC1131-100UG 04054839558016