Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MAB2148-C Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free)

This Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) is validated for use in flow cytometry, ICC, IP, IHC, ELISA & immunofluorescence for the detection of PECAM-1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: ECM Proteins Gene Symbol: PECAM1 UniProt Number: P16284
View Products on Sigmaaldrich.com
MAB2148-C
100 µg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFC, ICC, IP, IHC, ELISA, IFMPurifiedMonoclonal Antibody
Description
Catalogue NumberMAB2148-C
Replaces04-1074, MAB2148
DescriptionAnti-PECAM-1 Antibody, clone P2B1 (Ascites Free)
Alternate Names
  • Platelet endothelial cell adhesion molecule
  • PECAM-1
  • EndoCAM
  • GPIIA
  • PECA1
  • CD31
Background InformationPECAM-1 is a type I integral membrane glycoprotein, and a member of the immunoglobulin superfamily of cell surface receptors. PECAM-1 is constitutively expressed on the surface of endothelial cells where it is concentrated at cell junctions. PECAM-1 is also expressed at low levels on all leukocytes and platelets. Homotypic interaction of CD31 is important in cell-cell interaction, cell-matrix interaction and signal transduction.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-PECAM-1 Antibody, clone P2B1 (Ascites Free) is validated for use in flow cytometry, ICC, IP, IHC, ELISA & immunofluorescence for the detection of PECAM-1.
Key Applications
  • Flow Cytometry
  • Immunocytochemistry
  • Immunoprecipitation
  • Immunohistochemistry
  • ELISA
  • Immunofluorescence
Application NotesImmunocytochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in pluripotent cells (Boyd, N. L., et al. (2007). Exp Biol Med. 232(6):833-843.).

Immunoprecipitation Analysis: A representative lot detected PECAM-1 by immunoprecipitation.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).

ELISA Analysis: A representative lot of from an independent laboratory detected PECAM-1 in a panel of CD31 mutant cell lines (Newton, J. P, et al. (1997) J. Biol. Chem., 272: 20555-63.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected PECAM-1 in astrocytoma tissue sections (Bronger, H., et al. (2005). Cancer Res. 65(24):11419-28.).
Biological Information
ImmunogenWhole cells expressing human PECAM-1.
CloneP2B1
Concentration1 mg/mL
HostMouse
IsotypeIgG1κ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PECAM1
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~80 kDa calculated
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1 µg of this antibody detected PECAM-1 in 1X10E6 human PBMCs.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MAB2148-C 04053252975554