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AB3242 Anti-PGC-1 Antibody

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AB3242
100 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RELISA, ICC, IF, IHC, IP, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB3242
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-PGC-1 Antibody
Alternate Names
  • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
  • Ligand effect modulator 6
  • PGC-1-alpha
  • PPAR-gamma coactivator 1-alpha
  • PPARGC-1-alpha
Background InformationPGC-1 is a transcription factor that determines whether preadipocytes will differentiate into brown or white fat cells. It interacts with PPARgamma to promote the expression of a cassette of genes which define the brown adipocyte phenotype.
References
Product Information
FormatAffinity Purified
Control
  • BAT nuclear prep
PresentationPurified rabbit polyclonal antibody in buffer containing 20 mM PBS and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PGC-1 Antibody is a rabbit olyclponal antibody for the detection of PGC-1 also known as PPARgamma Coactivator 1-alpha & has been validated in ELISA, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, and Western Blotting.
Key Applications
  • ELISA
  • Immunocytochemistry
  • Immunofluorescence
  • Immunohistochemistry
  • Immunoprecipitation
  • Western Blotting
Application NotesELISA Analysis: A 1:75,000 dilution of a previous lot was used in ELISA.

Immunocytochemistry Analysis: A 1:300 dilution of a previous lot was used in Immunocytochemistry.

Immunofluorescence Analysis: Representative lots detected PGC-1α in frozen mouse and rat muscle tissue sections (Gouspillou, G., et al. (2013). Longev. Healthspan. 2(1):13; Matsuura, T., et al. (2007). J. Orthop. Res. 25(11):1534-1540).

Immunohistochemistry Analysis: A representative lot detected PGC-1α in free-floating mouse forebrain sections (Tallaksen-Greene, S.J., et al. (2014). J Neurosci. 34(47):15658-15668).

Immunoprecipitation Analysis: Representative lots immunoprecipitated PGC-1α from mouse liver, skeletal muscle, and brown adipose tissue lysates (Boutant, M., et al. (2016). Cell Rep. 14(9):2068-2075; Park, R.D., et al. (2014). J. Exerc. Nutrition Biochem. 18(1):1-7; Kim, J.S., et al. (2014). J. Exerc. Nutrition Biochem. 18(3):259-266; Philip, A., et al. (2011). J. Biol. Chem. 286(35):30561-30570).

Western Blotting Analysis: Representative lots detected similar PGC-1α in human, mouse, and rat skeletal muscle tissue, as well as in nuclear and mitochondrial preparations from rat brown adipose tissue (Suwa, M., et al. (2015). J. Sports Sci. Med. 14(3):548-555; Lee, I., et al. (2015). Front. Pharmacol. 6:43; Vincent, G., et al. (2015). Front Physiol. 6:51; Lombardi, A., et al. (2015). PLoS One. 10(2):e0116498; Gouspillou, G., et al. (2014). FASEB J. 28(4):1621-1633; Saleem, A., et al. (2014). Am. J. Physiol. Cell Physiol. 306(3):C241-249).
Biological Information
ImmunogenSynthetic peptide from amino acids 777-797 (C-terminus) of mouse PGC-1.
EpitopeC-terminus
HostRabbit
SpecificityImmunogen peptide blocking prior to Western blotting completely abolished target band detection by this rabbit polyclonal antibody.
Species Reactivity
  • Human
  • Mouse
  • Rat
Species Reactivity NoteHuman, Mouse, Rat.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity.
Gene Symbol
  • Ppargc1a
  • Pgc1
  • Pgc1a
  • Ppargc1
Purification MethodAffinity purified
UniProt Number
UniProt SummaryFUNCTION: Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis.
SIZE: 797 amino acids; 90,588 Da
SUBUNIT: Binds MYBBP1A, which inhibits transcriptional activation by this protein (By similarity). Interacts with LRPPRC. Homooligomer.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Brown adipose tissue, heart, kidney and brain.
SIMILARITY: Contains 1 RRM (RNA recognition motif) domain.
Molecular Weight~100 kDa observed. 91.03/89.64/91.71/77.14 kDa (human isoform 1/B4/B5/L-PGG-1alpha), 90.59 kDa (mouse isoform 1; PGC-1a1), 90.62 kDa (rat) calculated.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse BAT extracts.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected recombinant mouse PGC-1, as well as endogenous PGC-1 in 15 µg of nuclear extract and whole cell lysate from mouse brown adipose tissue (BAT). Immunogen peptide blocking completely blocked target band detection.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -70ºC from date of receipt in undiluted aliquots. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB3242 04053252320866

Documentation

Anti-PGC-1 Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-PGC-1 Antibody Certificates of Analysis

TitleLot Number
Anti-PGC-1 -
Anti-PGC-1 - 2122418 2122418
Anti-PGC-1 - 2373515 2373515
Anti-PGC-1 - 2392229 2392229
Anti-PGC-1 - 1972278 1972278
Anti-PGC-1 - 2021186 2021186
Anti-PGC-1 - 2039763 2039763
Anti-PGC-1 - 2181243 2181243
Anti-PGC-1 - 2309025 2309025
Anti-PGC-1 - 3170796 3170796

References

Reference overviewApplicationPub Med ID
Effects of Nitric Oxide Synthase Inhibition on Fiber-Type Composition, Mitochondrial Biogenesis, and SIRT1 Expression in Rat Skeletal Muscle.
Suwa, M; Nakano, H; Radak, Z; Kumagai, S
Journal of sports science & medicine  14  548-55  2015

Show Abstract
26336341 26336341
3,5-Diiodo-L-thyronine activates brown adipose tissue thermogenesis in hypothyroid rats.
Lombardi, A; Senese, R; De Matteis, R; Busiello, RA; Cioffi, F; Goglia, F; Lanni, A
PloS one  10  e0116498  2015

Show Abstract
25658324 25658324
Changes in mitochondrial function and mitochondria associated protein expression in response to 2-weeks of high intensity interval training.
Vincent, G; Lamon, S; Gant, N; Vincent, PJ; MacDonald, JR; Markworth, JF; Edge, JA; Hickey, AJ
Frontiers in physiology  6  51  2015

Show Abstract
25759671 25759671
A metabolic switch toward lipid use in glycolytic muscle is an early pathologic event in a mouse model of amyotrophic lateral sclerosis.
Palamiuc, L; Schlagowski, A; Ngo, ST; Vernay, A; Dirrig-Grosch, S; Henriques, A; Boutillier, AL; Zoll, J; Echaniz-Laguna, A; Loeffler, JP; René, F
EMBO molecular medicine  7  526-46  2015

Show Abstract
25820275 25820275
(-)-Epicatechin combined with 8 weeks of treadmill exercise is associated with increased angiogenic and mitochondrial signaling in mice.
Lee, I; Hüttemann, M; Kruger, A; Bollig-Fischer, A; Malek, MH
Frontiers in pharmacology  6  43  2015

Show Abstract
Western Blotting25821434 25821434
p53 is necessary for the adaptive changes in cellular milieu subsequent to an acute bout of endurance exercise.
Saleem, A; Carter, HN; Hood, DA
American journal of physiology. Cell physiology  306  C241-9  2014

Show Abstract
Western Blotting24284795 24284795
Increased sensitivity to mitochondrial permeability transition and myonuclear translocation of endonuclease G in atrophied muscle of physically active older humans.
Gouspillou, Gilles, et al.
FASEB J., 28: 1621-33 (2014)  2014

Show Abstract
24371120 24371120
PARK2/Parkin-mediated mitochondrial clearance contributes to proteasome activation during slow-twitch muscle atrophy via NFE2L1 nuclear translocation.
Furuya, N; Ikeda, S; Sato, S; Soma, S; Ezaki, J; Oliva Trejo, JA; Takeda-Ezaki, M; Fujimura, T; Arikawa-Hirasawa, E; Tada, N; Komatsu, M; Tanaka, K; Kominami, E; Hattori, N; Ueno, T
Autophagy  10  631-41  2014

Show Abstract
24451648 24451648
The relationship between muscle fiber type-specific PGC-1α content and mitochondrial content varies between rodent models and humans.
Gouspillou, G; Sgarioto, N; Norris, B; Barbat-Artigas, S; Aubertin-Leheudre, M; Morais, JA; Burelle, Y; Taivassalo, T; Hepple, RT
PloS one  9  e103044  2014

Show Abstract
25121500 25121500
Dynamic partnership between TFIIH, PGC-1α and SIRT1 is impaired in trichothiodystrophy.
Traboulsi, H; Davoli, S; Catez, P; Egly, JM; Compe, E
PLoS genetics  10  e1004732  2014

Show Abstract
25340339 25340339