Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABF2071-25UG Anti-PLVAP Antibody, clone MECA-32

Anti-PLVAP, clone MECA-32, Cat. No. MABF2071, is a rat monoclonal antibody tht detects Plasmalemma vesicle-associated protein (PV_1) and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

Anti-PLVAP Antibody, clone MECA-32 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Plvap, Pv1 UniProt Number: Q91VC4
View Products on Sigmaaldrich.com
MABF2071-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MIH(P), IP, WBRPurifiedMonoclonal Antibody
Description
Catalogue NumberMABF2071-25UG
DescriptionAnti-PLVAP Antibody, clone MECA-32
Alternate Names
  • Plasmalemma vesicle-associated protein
  • MECA-32 antigen
  • Plasmalemma vesicle protein 1
  • PV-1
Background InformationPlasmalemma vesicle-associated protein (UniProt: Q91VC4; also known as MECA-32 antigen, Plasmalemma vesicle protein 1, PV-1) is encoded by the Plvap (also known as Pv1) gene (Gene ID: 84094) in murine species. MECA-32 is a single-pass type II membrane protein that is involved in the formation of stomatal and fenestral diaphragms of caveolae and may also function in microvascular permeability. It is expressed in lung, kidney, spleen, heart, muscle, eye, pancreas, thyroid, thymus, submaxillary gland, prostate, epididymis, uterus, and liver. MECA-32 contains three coiled coil regions (aa 140-160; 189-224; and 281-383). It has a short cytoplasmic domain (aa 1-26), a transmembrane domain (aa 27-47), and a long extracellular domain (aa 48-438). In mouse embryo it is expressed at 7 days post coitum (dpc). Its expression in the brain its expression is negatively correlated with the differentiation of the vasculature to form the blood brain barrier. Between 12 to 16 dpc higher expression is observed in endothelial cell surfaces of blood vessels in the brain. (Ref.: Hallmann, R., et al. (1995). Dev. Dynamics. 202; 525-332).
References
Product Information
FormatPurified
PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-PLVAP, clone MECA-32, Cat. No. MABF2071, is a rat monoclonal antibody tht detects Plasmalemma vesicle-associated protein (PV_1) and has been tested for use in Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
Key Applications
  • Immunohistochemistry (Paraffin)
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunohistochemistry Analysis: A representative lot detected PLVAP in Immunohistochemistry applications (Tanneau, G.M., et. al. (1999). J Histochem Cytochem. 47(12):1581-92; Hallmann, R., et. al. (1995). Dev Dyn. 202(4):325-32).

Immunohistochemistry Analysis: A representative lot detected PLVAP in Immunohistochemistry applications (Kopp, H.G., et. al. (2005). Blood. 106(2):505-13).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PLVAP in mouse liver tissue.

Immunoprecipitation Analysis: A representative lot detected PLVAP in Immunoprecipitation applications (Hallmann, R., et. al. (1995). Dev Dyn. 202(4):325-32).

Western Blotting Analysis: A representative lot detected PLVAP in Western Blotting applications (Hallmann, R., et. al. (1995). Dev Dyn. 202(4):325-32).
Biological Information
ImmunogenMouse lymph node stromal cells.
CloneMECA-32
ConcentrationPlease refer to lot specific datasheet.
HostRat
SpecificityClone MECA-32 is a rat monoclonal antibody that detects Plasmalemma vesicle-associated protein (PLVAP) in murine species.
IsotypeIgG2aκ
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • Plvap
  • Pv1
Purification MethodProtein G purified
UniProt Number
Molecular Weight50- 55 kDa observed; 49.93 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PLVAP in mouse kidney tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2071-25UG 04054839431821