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04-639 Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12

This Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12 is validated for use in WB for the detection of PP2A.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Kinases & Phosphatases Gene Symbol: PPP2R5D, B56D, MGC2134, MGC8949 UniProt Number: Q14738
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04-639
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue Number04-639
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-PP2A Antibody, B subunit, B56 delta, clone H5D12
Background InformationPP2A is a heterotrimeric phosphatase, ccomposed of A, B and C subunits. The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. Three families of B subunits have been identified thus far. The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variantsderived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG1 antibody in PBS with 0.05% NaN3.
Quality LevelMQ100
Applications
ApplicationThis Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12 is validated for use in WB for the detection of PP2A.
Key Applications
  • Western Blotting
Application NotesImmunoblotting (1:500 to 1:2000)

Optimal working dilutions must be determined by the end user
Biological Information
ImmunogenGST fusion protein corresponding to the N-terminus of human PP2A, B subunit, B56 delta
CloneH5D12
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityHuman PP2A, B subunit, B56 delta. This monoclonal antibody is the delta isoform for the B56 family of protein phosphatase 2A.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Entrez Gene SummaryThe product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an delta isoform of the regulatory subunit B56 subfamily.
Gene Symbol
  • PPP2R5D
  • B56D
  • MGC2134
  • MGC8949
UniProt Number
UniProt SummaryFUNCTION: The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
TISSUE SPECIFICITY: Isoform Delta-2 is widely expressed. Isoform Delta-1 is highly expressed in brain.
INDUCTION: By retinoic acid; in neuroblastoma cell lines.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the phosphatase 2A regulatory subunit B56 family.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
04-639 04053252584572

Documentation

Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12 SDS

Title

Safety Data Sheet (SDS) 

Anti-PP2A Antibody, B subunit, B56 delta, clone H5D12 Certificates of Analysis

TitleLot Number
Anti- PP2A, B subunit, B56 delta, clone H5D12 - NG1524691 NG1524691
Anti- PP2A, B subunit, B56 delta, clone H5D12 -2765920 2765920
Anti- PP2A, B subunit, B56 delta, clone H5D12 -3167166 3167166
Anti- PP2A, B subunit, B56 delta, clone H5D12 Monoclonal Antibody 2876059
Anti- PP2A, B subunit, B56 delta, clone H5D12_2845536 2845536