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ABS2123-25UL Anti-PPOX

Anti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.

Research Category: Signaling Gene Symbol: PPOX UniProt Number: P50336
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ABS2123-25UL
25 µL  
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Overview

Replacement Information

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
MWBRbUnpurifiedPolyclonal Antibody
Description
Catalogue NumberABS2123-25UL
DescriptionAnti-PPOX
Alternate Names
  • Protoporphyrinogen oxidase
  • PPOX
Background InformationProtoporphyrinogen oxidase (UniProt: P50336; also known as EC: 1.3.3.4, PPO, PPOX) is encoded by the PPOX gene (Gene ID: 5498) in human. Protoporphyrinogen oxidase is a mitochondrial inner membrane enzyme that catalyzes the 6-electron oxidation of protoporphyrinogen-IX to form protoporphyrin-IX. It is involved in step one of the subpathway that synthesizes protoporphyrin-IX from protoporphyrinogen-IX. It is expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, and pancreas and may be present as a monomer or active homodimeric form. Defects in PPOX are linked to variegate porphyria that lead to severe deficiency of this enzyme that result in the accumulation and increased excretion of porphyrins or porphyrin precursors. They are classified as erythropoietic or hepatic, depending on whether the enzyme deficiency occurs in red blood cells or in the liver.
References
Product Information
FormatUnpurified
PresentationRabbit polyclonal antiserum with 0.05% sodium azide.
Applications
ApplicationAnti-PPOX, Cat. No. ABS2123, is a rabbit polyclonal antibody that detects Protoporphyrinogen oxidase and has been tested for use in Western Blotting.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: A representative lot detected PPOX in undifferentiated (day 0) and maturing (day 3 or 4) mouse erythroleukemia (MEL) cells (Chung, J., et. al. (2015). Sci Signal. 8(372):ra34). and differentiated Mouse erythroleukemia (MEL) cells (Medlock, A.E., et. al. (2015). PLoS One. 10(8):e0135896).
Biological Information
ImmunogenFull length recombinant human Protoporphyrinogen oxidase (PPOX).
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects Protoporphyrinogen oxidase (PPO) in Human and Mouse cells.
IsotypeIgG
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Gene Symbol
  • PPOX
Purification MethodUnpurified
UniProt Number
Molecular Weight~53 kDa observed; 50.77 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lystate from mitochondria isolated from differentiated DS19 cells.

Western Blotting Analysis: A 1:500 dilution of this antibody detected PPOX in lystate from mitochondria isolated from differentiated DS19 cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Packaging Information
Material Size25 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABS2123-25UL 04054839505546

Documentation