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05-1570 Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3

Use Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.

Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function, Inflammation & Immunology Research Sub-Category: Transcription Factors, Transcription Factors Gene Symbol: BLIMP1, PRDM1, PRDI-BF1, BLIMP-1 UniProt Number: Q60636
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05-1570
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, ELISA, IHC, FCRPurifiedMonoclonal Antibody
Description
Catalogue Number05-1570
DescriptionAnti-PRDM1 (BLIMP-1) Antibody, clone 6D3
Alternate Names
  • PR domain containing 1, with ZNF domain
  • PR-domain zinc finger protein 1
  • beta-interferon gene positive-regulatory domain I binding factor
  • Positive regulatory domain I-binding factor 1
  • PRDI-binding factor-1
  • Beta-interferon gene positive regulatory domain I-binding factor
  • B-lymphocyte-induced maturation protein 1
  • PR domain-containing protein 1
  • PRDI-binding factor 1
  • PR domain zinc finger protein 1
Background InformationB-lymphocyte induced maturation protein (BLIMP-1) is a 100 kDa transcriptional repressor containing 1 DET domain and 4 C2H2-type zinc fingers. In the nucleus, BLIMP-1 is known to bind specifically with the Beta-interferon gene positive regulatory domain I (PRDI), and to interact with PRMT5. During B-cell differentiation, BLIMP-1 actively represses the transcription of the gene, c-myc, through binding its promoter and has been deemed a “master regulator” for terminal B-cell differentiation, as it is able to initiate the BCL1 cell developmental cascade.
References
Product Information
FormatPurified
Control
  • HL-60 cell lysate
PresentationPurified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationUse Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 (Rat Monoclonal Antibody) validated in WB, ELISA, IHC, FC to detect PRDM1 (BLIMP-1) also known as PR-domain zinc finger protein 1.
Key Applications
  • Western Blotting
  • ELISA
  • Immunohistochemistry
  • Flow Cytometry
Application NotesELISA Analysis: A representative lot was used by an independent laboratory in ELISA. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)

Flow Cytometry Analysis: A representative lot was used by an independent laboratory in FC. (Kallies, A., et al. (2004). J Exp Med. 200(8):967-977.)
Biological Information
ImmunogenRecombinant protein corresponding to mouse BLIMP-1.
EpitopeRecognizes epitope located between amino acids 255-395 of BLIMP-1
Clone6D3
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRat
SpecificityRecognizes BLIMP-1
IsotypeIgG2aκ
Species Reactivity
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • BLIMP1
  • PRDM1
  • PRDI-BF1
  • BLIMP-1
Purification MethodProtein G
UniProt Number
UniProt SummaryFUNCTION: Transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene. Drives the maturation of B-lymphocytes into Ig secreting cells.

SUBUNIT STRUCTURE: Interacts with PRMT5. Ref.5

SUBCELLULAR LOCATION: Nucleus (By similarity).

SEQUENCE SIMILARITIES: Contains 4 C2H2-type zinc fingers.

Contains 1 SET domain.

SEQUENCE CAUTION: The sequence AAI29802.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
Molecular Weight~ 95 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected BLIMP-1 on 10 µg of HL-60 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-1570 04053252641367

Documentation

Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 SDS

Title

Safety Data Sheet (SDS) 

Anti-PRDM1 (BLIMP-1) Antibody, clone 6D3 Certificates of Analysis

TitleLot Number
Anti-PRDM1 (BLIMP-1), clone 6D3 - 2436811 2436811
Anti-PRDM1 (BLIMP-1), clone 6D3 - 2036705 2036705
Anti-PRDM1 (BLIMP-1), clone 6D3 - 2318058 2318058
Anti-PRDM1 (BLIMP-1), clone 6D3 - 3385828 3385828
Anti-PRDM1 (BLIMP-1), clone 6D3 - 3601734 3601734
Anti-PRDM1 (BLIMP-1), clone 6D3 - 3720636 3720636
Anti-PRDM1 (BLIMP-1), clone 6D3 - 3827474 3827474
Anti-PRDM1 (BLIMP-1), clone 6D3 - 4109604 4109604
Anti-PRDM1 (BLIMP-1), clone 6D3 - NG1926423 NG1926423
Anti-PRDM1 (BLIMP-1), clone 6D3 - NRG1771415 NRG1771415
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