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MABE629

Sigma-Aldrich

Anti-PRKDC, clone 3H6 Antibody

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

antibody form

ascites fluid

Quality Level

clone

3H6
monoclonal

purified by

(unpurified)

species reactivity

mouse, human

concentration

(Please refer to lot specific datasheet.)

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

UniProt accession no.

General description

Purified recombinant fragment of human PRKDC expressed in E. Coli.

Application

Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected PRKDC in breast cancer, colon cancer, lung cancer, and brain tissues.

Immunocytochemistry Analysis (ICC): A 1:200-1,000 dilution from a representative lot detected PRKDC in HeLa cells.

ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Quality

Evaluated by Western Blotting in HEK293 and PRKDC -- hIgGFc transfected HEK293 cell lysates.

Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected PRKDC in HEK293 and hIgGFc transfected HEK293 cell lysates.

Physical properties

~47 kDa observed. Uncharacterized bands may be observed in some lysate(s).

Physical form

Mouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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