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MABS450B-25UG Anti-PTEN-Long Antibody, clone 1C2 Antibody, biotin conjugate

Anti-PTEN-Long, clone 1C2, biotin conjugate, Cat. No. MABS450B, is a mouse monoclonal antibody that detects PTEN-Long and is tested for use in ELISA and Western Blotting.

MABS450B-25UG
25 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABS450B-25UG
DescriptionAnti-PTEN-Long Antibody, clone 1C2 Antibody, biotin conjugate
Alternate Names
  • Phosphatidylinositol 3
  • 4
  • 5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN
  • EC:3.1.3.16
  • Mutated in multiple advanced cancers 1
  • Phosphatase and tensin homolog
Background InformationPhosphatidylinositol 3,4,5-trisphosphate 3-phosphatase and dual-specificity protein phosphatase PTEN (UniProt: P60484; also known as Mutated in multiple advanced cancers 1, Phosphatase and tensin homolog, PTEN) is encoded by the PTEN (also known as MMAC1, TEP1) gene (Gene ID: 5728) in human. PTEN belongs to the family of lipid phosphatases. It contains an N-terminal phosphatase catalytic domain, a C2-domain that binds to phospholipid membranes, and a C-terminal regulatory tail. It is a dual-specific phosphatase, which dephosphorylates both lipid and protein substrates. PTEN blocks the phosphatidylinositol 3-kinase/Akt signaling pathway by removing the phosphate in the 3-position on phosphoinositide-3, 4, 5-trisphosphate (PIP3). Dephosphorylation of PIP3 is an important factor in cell growth, proliferation, apoptosis, and survival. Dephosphorylation of PIP3 blocks Akt signaling that can lead to higher activity of pro-apoptotic molecules such as Bad and Caspase-9. PTEN activity, stability, localization, and conformation are controlled through complex regulatory mechanisms, including the reversible phosphorylations of several Serine/Threonine residues in its unfolded C-terminal tail region (PTEN-C-tail, residues 351-403). Alternative translation of PTEN is reported to encode a 576-amino acid translational variant that is known as PTEN-Long with a 173-amino acid domain at its N-terminus followed by the classical 403 amino acids of PTEN. PTEN-Long is a membrane permeable, secreted protein that can enter other cells and acts as an antagonist of the PI3K pathway. PTEN is considered a major tumor suppressor and its mutations are reported in several human tumors. Over seventy different types of PTEN mutation have been recorded in patients with Cowden syndrome. PTEN -/- mice exhibit higher levels of 3 -phosphorylated phospholipids and die during embryogenesis due to the failure of developmental apoptosis. Clone 1C2 specifically binds to PTEM-Long. It can be used in conjunction with clone 1A1 where clone 1A captures both PTEN and PTEN-Long and clone 1C2 can be used to detect PTEN-Long. (Ref.: Hopkins, BD., et al. (2013). Science. 341(6144); 399-402).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-PTEN-Long, clone 1C2, biotin conjugate, Cat. No. MABS450B, is a mouse monoclonal antibody that detects PTEN-Long and is tested for use in ELISA and Western Blotting.
Key Applications
  • ELISA
  • Western Blotting
Application NotesWestern Blotting Analysis: 1:250 dilution from a representative lot detected PTEN-Long in HEK293 cell lysates.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenA bacterial fusion protein with a V5/His-tag at its C- terminus.
EpitopeN-terminal half
Clone1C2
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1C2 is a mouse monoclonal antibody that detects PTEN-Long.
IsotypeIgG1κ
Species Reactivity
  • Human
Species Reactivity NoteHuman.
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PTEN
  • MMAC1
  • TEP1
Purification MethodProtein G purified
UniProt Number
Molecular Weight~75 kDa observed; 64.88 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by ELISA in PTEN fusion protein.

ELISA Analysis: Various dilutions (starting at 5 mg/mL, two-fold serial dilutions; 6 points) of this antibody detected PTEN fusion protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABS450B-25UG 04061841879725