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MABE978 Anti-Paired box protein Pax-1 Antibody, clone 7F11.2

This Anti-Paired box protein Pax-1 Antibody, clone 7F11.2 is validated for use in Western Blotting for the detection of Paired box protein Pax-1.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Nuclear Receptors Gene Symbol: PAX1, HUP48 UniProt Number: P15863
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MABE978
100 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMPurifiedMonoclonal Antibody
Description
Catalogue NumberMABE978
DescriptionAnti-Paired box protein Pax-1 Antibody, clone 7F11.2
Alternate Names
  • HuP48
  • Paired box protein Pax-1
Background InformationPaired box protein Pax-1 (UniProt P15863; also known as HuP48) is encoded by the PAX1 (also known as HUP48, OFC2) gene (Gene ID 5075) in human. Pax-1 belongs to the family of transcription factors with conserved DNA-binding paired box domain. The family of paired box (PAX) genes are divided into subgroup I (PAX1 and PAX9), II (PAX2, PAX5, and PAX8), III (PAX3 and PAX7), and IV (PAX4 and PAX6). Pax1 & Pax9 are paralogous genes with overlapping expression and function. Pax1 is required for the proper development of the ventral structures of the vertebral column and double homozygous mutants of Pax1 and Pax9 result in a dramatic increase in the severity of the vertebral defects. During the early stages of chondrogenic differentiation, Pax1 induces and transactivates the transcriptional repressor Nk3 homeobox 2 (Nkx3.2) that promotes chondrogenesis of sclerotomal cells, while Pax1 act as a negative regulator during chondrogenic differentiation by antagonizing against Sox9-driven chondrocyte maturation. Pax1 is downregulated as cartilage formation proceeds and becomes restricted to the mesenchymal condensations that give rise to the anlagen of the intervertebral discs, and to the perichondrium surrounding the cartilaginous vertebral bodies.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal IgG2bκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationThis Anti-Paired box protein Pax-1 Antibody, clone 7F11.2 is validated for use in Western Blotting for the detection of Paired box protein Pax-1.
Key Applications
  • Western Blotting
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to the N-terminal half of human Paired box protein Pax-1.
EpitopeN-terminal half
Clone7F11.2
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityReactivity toward spliced isoforms 2 and 3 has not been determined.
IsotypeIgG2bκ
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • PAX1
  • HUP48
Purification MethodProtein G Purified
UniProt Number
Molecular Weight~58 kDa observed. Uncharacterized band(s) may appear in some lysates.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Paired box protein Pax-1 in 10 µg of K562 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABE978 04055977294323