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05-321MG Anti-Phosphotyrosine Antibody, clone 4G10®

Anti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.

Anti-Phosphotyrosine Antibody, clone 4G10® MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: General Post-translation Modification
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05-321MG
1 mg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
AIP, WBMPurifiedMonoclonal Antibody
Description
Catalogue Number05-321MG
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-Phosphotyrosine Antibody, clone 4G10®
Background InformationSome of the tyrosine residues can be tagged with a phosphate group (phosphorylated) by protein kinases. (In its phosphorylated state, it is referred to as phosphotyrosine.). Tyrosine phosphorylation is considered as one of the key steps in signal transduction and regulation of enzymatic activity.
The advent of anti-phosphotyrosine antibodies is one of significant events in signal transduction research. Before the availability of anti-phosphotyrosine antibodies, tyrosyl phospyhorylation of proteins and enzymes was investigated through hazardous and time-consuming radioactive experiments. Anti-phosphotyrosine antibodies are commonly used in western blots after the targeted proteins have been immunoprecipitated to measure the tyrosyl phosphorylation of the proteins. Anti-phosphotyrosine antibodies are also directly used on cell lysate to examine the overall change of tyrosine phosphorylation level in reponse to various treatments.
References
Product Information
FormatPurified
Control
  • Untreated A-431 (negative control) and EGF treated A-431 (positive control) whole cell lysates.
    Included Positive Antigen Control: Catalog # 12-302, EGF-stimulated A431 cell lysate is provided as a free positive antigen control for western immunoblotting. Aliquot as desired, refreeze immediately, and store at -20°C. The lysate is stable for 6 months at -20°C. Before use, add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for immunoblot analysis.
PresentationPurified mouse monoclonal IgG2bκ in buffer containing PBS, pH 7.4. Liquid at 2-8ºC.
IgG2bκ mouse monoclonal antibody produced in vitro by mouse-mouse hybridoma 4G10® (FOX-NY [NS-1 derivative] myeloma x spleen cells).
Quality LevelMQ100
Applications
ApplicationAnti-Phosphotyrosine Antibody, clone 4G10 detects level of Phosphotyrosine & has been published & validated for use in IP & WB.
Key Applications
  • Immunoprecipitation
  • Western Blotting
Application NotesImmunoprecipitation:
2-4 μg of a previous lot immunoprecipitated quantitatively the phosphotyrosine containing proteins in the lysate of a confluent culture (10 cm dish) of cells expressing an activated tyrosine kinase. To preserve phosphotyrosine, add: 0.2 mM sodium orthovanadate to the lysis buffer.
Biological Information
ImmunogenPhosphotyramine-KLH
Clone4G10®
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostMouse
SpecificityThis antibody recognizes tyrosine-phosphorylated proteins from all species.
IsotypeIgG2bκ
Species Reactivity
  • All
Antibody TypeMonoclonal Antibody
Modifications
  • Phosphorylation
Purification MethodProtein G-Sepharose™ Chromatography
Molecular WeightDependent upon the molecular weight of the tyrosine phosphorylated protein being detected.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated on EGF-treated human A431 carcinoma cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected tyrosine-phosphorylated proteins in a modified RIPA lysate from EGF-treated human A431 carcinoma cells (Cohen, B., 1990; Druker, B. J., 1989; Kanakura, Y., 1991).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.

NOTE: DO NOT FREEZE.
For maximum recovery of the product, centrifuge the original vial prior to removing the cap. If the product has accidentally been frozen and thawed, spin it at 13,000 x g for 10 minutes at 2-8ºC. Save the supernatant for application.
Packaging Information
Material Size1 mg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-321MG 04053252370953

Documentation

Anti-Phosphotyrosine Antibody, clone 4G10® SDS

Title

Safety Data Sheet (SDS) 

Anti-Phosphotyrosine Antibody, clone 4G10® Certificates of Analysis

TitleLot Number
Anti-Phosphotyrosine, clone 4G10 (mouse monoclonal IgG2bk) - DAM1400107 DAM1400107
Anti-Phosphotyrosine, clone 4G10#174; - 2040085 2040085
Anti-Phosphotyrosine, clone 4G10#174; - 704057853 704057853
Anti-Phosphotyrosine, clone 4G10#174; - DAM1400107 DAM1400107
Anti-Phosphotyrosine, clone 4G10#174; - DAM1707888 DAM1707888
Anti-Phosphotyrosine, clone 4G10#174; - DAM1724014 DAM1724014
Anti-Phosphotyrosine, clone 4G10#174; - DAM1747347 DAM1747347
Anti-Phosphotyrosine, clone 4G10#174; - DAM1780352 DAM1780352
Anti-Phosphotyrosine, clone 4G10#174; - JBC1878055 JBC1878055
Anti-Phosphotyrosine, clone 4G10reg; - 29616 29616
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Related Products & Applications

Alternative Packsize

Catalog Number Description
05-321 Anti-Phosphotyrosine Antibody, clone 4G10®
05-321X Anti-Phosphotyrosine Antibody, clone 4G10®

Alternative Format

Catalog Number Description
05-777 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®
16-101 Anti-Phosphotyrosine Antibody, clone 4G10®, agarose conjugate
16-103 Anti-Phosphotyrosine Antibody, clone 4G10®, Biotin Conjugate
16-104 Anti-Phosphotyrosine Antibody, clone 4G10®, FITC conjugate
16-105 Anti-Phosphotyrosine Antibody, clone 4G10®, HRP conjugate
16-199 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, agarose conjugate
16-204 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, biotin conjugate
16-205 Anti-Phosphotyrosine Antibody, recombinant clone 4G10®, FITC conjugate

Included Positive Control

Catalog Number Description
12-302 EGF-Stimulated A431 Cell Lysate

Product Families

Phosphotyrosine Signaling Solutions

Study tyrosine phosphorylation using Millipore's specific and sensitive antibodies, including the popular clone 4G10®, and new 4G10 Platinum, available in various conjugations. See below for more.
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Phosphotyrosine Antibodies

Millipore’s phosphotyrosine antibodies, including the popular 4G10 clone, demonstrate sensitivity and detect multiple tyrosine phosphorylations on numerous substrates. See below for pTyr antibodies.
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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies