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AB5589-200UL Anti-Potassium Channel Kv1.3 Antibody, extracellular domain

This Anti-Potassium Channel Kv1.3 Antibody, extracellular domain is validated for use in FC, WB for the detection of Potassium Channel Kv1.3.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Ion Channels & Transporters Gene Symbol: KCNA3, HLK3, HGK5, KV1.3, HuKIII, HPCN3, Kv1.3, MK3, HUKIII, PCN3 UniProt Number: P22001
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AB5589-200UL
200 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RFC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5589-200UL
Brand Family Chemicon®
Trade Name
  • Chemicon
DescriptionAnti-Potassium Channel Kv1.3 Antibody, extracellular domain
References
Product Information
FormatAffinity Purified
Control
  • Included free of charge with the antibody is 40 μg of control antigen peptide (lyophilized powder). For negative control, preincubate 5 μg of the control antigen peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
PresentationAffinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Quality LevelMQ100
Applications
ApplicationThis Anti-Potassium Channel Kv1.3 Antibody, extracellular domain is validated for use in FC, WB for the detection of Potassium Channel Kv1.3.
Key Applications
  • Flow Cytometry
  • Western Blotting
Application NotesWestern blotting: 1:200-1:500 using ECL on rat brain membranes.

Indirect flow cytometry: 0.5-5 μg of antibody per 1x10e6cells.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.
Biological Information
ImmunogenHighly purified peptide corresponding to residues 263-275 of human Kv1.3 (Accession P22001).
Epitopeextracellular domain
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
SpecificityRecognizes Kv1.3, extracellular (Kcna3).
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Entrez Gene SummaryPotassium channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shaker-related subfamily. This member contains six membrane-spanning domains with a shaker-type repeat in the fourth segment. It belongs to the delayed rectifier class, members of which allow nerve cells to efficiently repolarize following an action potential. It plays an essential role in T-cell proliferation and activation. This gene appears to be intronless and it is clustered together with KCNA2 and KCNA10 genes on chromosome 1.
Gene Symbol
  • KCNA3
  • HLK3
  • HGK5
  • KV1.3
  • HuKIII
  • HPCN3
  • Kv1.3
  • MK3
  • HUKIII
  • PCN3
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P22001 # Mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.
SIZE: 523 amino acids; 58304 Da
SUBUNIT: Heterotetramer of potassium channel proteins. Binds PDZ domains of DLG1, DLG2 and DLG4 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
DOMAIN: SwissProt: P22001 The N-terminus may be important in determining the rate of inactivation of the channel while the tail may play a role in modulation of channel activity and/or targeting of the channel to specific subcellular compartments. & The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel family. A (Shaker) subfamily.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Packaging Information
Material Size200 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5589-200UL 04053252740565

Documentation

Anti-Potassium Channel Kv1.3 Antibody, extracellular domain SDS

Title

Safety Data Sheet (SDS) 

Anti-Potassium Channel Kv1.3 Antibody, extracellular domain Certificates of Analysis

TitleLot Number
RABBIT ANTI-Kv1.3, EXTRACELLULAR - 3856678 3856678
RABBIT ANTI-Kv1.3, EXTRACELLULAR - 3861345 3861345
RABBIT ANTI-Kv1.3, EXTRACELLULAR - 3899513 3899513
RABBIT ANTI-Kv1.3, EXTRACELLULAR - 3921945 3921945
RABBIT ANTI-Kv1.3, EXTRACELLULAR - 4059953 4059953
RABBIT ANTI-Kv1.3, EXTRACELLULAR - 4104147 4104147
RABBIT ANTI-Kv1.3, EXTRACELLULAR - 4164542 4164542
RABBIT ANTI-Kv1.3, EXTRACELLULAR -2782861A 2782861A
RABBIT ANTI-Kv1.3, EXTRACELLULAR AFFINITY PURIFIED - 3045098 3045098
RABBIT ANTI-Kv1.3, EXTRACELLULAR AFFINITY PURIFIED POLYCLONAL ANTIBODY 2876850
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