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ABT257 Anti-Pro-Collagen Type I, A1/COL1A1

Anti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.

Anti-Pro-Collagen Type I, A1/COL1A1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: COL1A1 UniProt Number: P02452
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ABT257
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, MWB, FC, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABT257
DescriptionAnti-Pro-Collagen Type I, A1/COL1A1
Alternate Names
  • Pro-collagen alpha-1(I) chain
  • Pro-Alpha-1 type I collagen
Background InformationCollagen alpha-1(I) chain (UniProt P02452; also known as Alpha-1 type I collagen) is encoded by the COL1A1 gene (Gene ID 1277) in human. Collagen is the major component of the extracellular matrix (ECM) and form the fibrils of tendons, ligaments, and bones. Type I collagen consists of two alpha I chains and one alpha 2 chain. Alpha-1 type I collagen is initially produced as an 1464-amino acid prepro-form with a signal peptide sequence (a.a. 1-22) and two propeptide sequences (a.a. 23-161 and a.a. 1219 –1464), the removal of which yields the mature alpha-1(I) chain. The mature alpha-1(I) chain is composed mostly of a large triple-helical region (a.a. 179-1192) sandwiched between two nonhelical segments known as the N-terminal telopeptide (a.a. 162-178; numbering based on the prepro-form) and the C-terminal telopeptide (a.a. 1193-1218; numbering based on the prepro-form). Collagen can be extracted from tissue via either enzymatic or non-enzymatic means. Collagen extracted using the proteolytic enzyme pepsin corresponds to the large triple-helical region, referred to as atelocollagen because both the N- and C-terminal telopeptides have been cleaved off by pepsin. On the other hand, collagen preparations obtained with non-enzymatic means (e.g. by acid extraction) have the intact telopeptides at both ends.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Pro-Collagen Type I Antibody is an antibody against Pro-Collagen Type I for use in Western Blotting, Flow Cytometry, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Flow Cytometry
  • Immunohistochemistry
Application NotesWestern Blotting Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung and skin tissue lysate.
Flow Cytometry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Immunohistochemistry Analysis: A representative lot detected Pro-Collagen Type I, A1/COL1A1 in mouse lung tissue.
Biological Information
ImmunogenLinear peptide corresponding to sequence derived from human Pro-Collagen Type I, A1/COL1A1 C-terminal propeptide region.
EpitopeC-terminal propeptide region
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
SpecificityCat. No. ABT257 Anti-Pro-Collagen Type I, A1/COL1A1 was raised against a sequence derived from the C-terminal propeptide (not the N-terminal propeptide) region. This polyclonal antibody will detect prepro- and pro-, but not mature collagen alpha-1(I) chain.
Species Reactivity
  • Human
  • Mouse
Species Reactivity NoteHuman, Mouse. Predicted to react with a broad range of species based on high sequence homology, including mammalian, avian, fish, and amphibian species.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • COL1A1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~140 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse skin tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Pro-Collagen Type I, A1/COL1A1 in 10 µg of mouse skin tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABT257 04055977136678

Documentation

Anti-Pro-Collagen Type I, A1/COL1A1 Certificates of Analysis

TitleLot Number
Anti-Pro-Collagen Type I, A1/COL1A1 2902699
Anti-Pro-Collagen Type I, A1/COL1A1 - 3185049 3185049
Anti-Pro-Collagen Type I, A1/COL1A1 - 3201707 3201707
Anti-Pro-Collagen Type I, A1/COL1A1 - 3225879 3225879
Anti-Pro-Collagen Type I, A1/COL1A1 - 3268885 3268885
Anti-Pro-Collagen Type I, A1/COL1A1 - 3275493 3275493
Anti-Pro-Collagen Type I, A1/COL1A1 - 3389910 3389910
Anti-Pro-Collagen Type I, A1/COL1A1 - 3596724 3596724
Anti-Pro-Collagen Type I, A1/COL1A1 - 3729946 3729946
Anti-Pro-Collagen Type I, A1/COL1A1 - 3777980 3777980
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Technical Info

Title
White Paper: Further considerations of antibody validation and usage.

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Categories

Life Science Research > Antibodies and Assays > Primary Antibodies