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07-1781 Anti-RAD50 Antibody

Use Anti-RAD50 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB to detect RAD50 also known as RAD50 (S. cerevisiae) homolog.

Anti-RAD50 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: EC 3.6.-.-, OTTHUMP00000065928, RAD50-2, hRAD50, hRad50 UniProt Number: Q92878
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07-1781
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RICC, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue Number07-1781
DescriptionAnti-RAD50 Antibody
Alternate Names
  • RAD50 (S. cerevisiae) homolog
  • RAD50 homolog
  • RAD50 homolog (S. cerevisiae)
Background InformationRAD50 (previously known as DNA repair protein RAD50) is homologous to Saccharomyces cerevisiae Rad50 and is a member of the structural maintenance of chromosome (SMC) family and is involved in cell growth and viability. RAD50 is known to encode 2 distinct proteins. The RAD50, XRS2/NBS1 complex is required for activation of ATM which will phosphorylate TERF1, thereby promoting telomerase. Along with MRE11 and NBN, RAD50 also forms the MRE11–RAD50–NBS1 (MRN) complex, which is one of the first factors to be localized to the DNA lesion, where it might initially have a structural role by tethering together, and therefore stabilizing, broken chromosomes. MRN also controls ATR, which is required for SMC1A phosphorylation, resulting in chromatid adhesion and DNA damage response. RAD50 is expressed in all tissue but predominantly in the testis and fibroblasts.
References
Product Information
FormatAffinity Purified
Control
  • Human spleen lysate.
PresentationPurified rabbit polyclonal antibody precipitated in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives.
Quality LevelMQ100
Applications
ApplicationUse Anti-RAD50 Antibody (Rabbit Polyclonal Antibody) validated in ICC, WB to detect RAD50 also known as RAD50 (S. cerevisiae) homolog.
Key Applications
  • Immunocytochemistry
  • Western Blotting
Application NotesImmunocytochemistry:
A 1:500 dilution of this antibody was used to detect RAD50 on HeLa and A431 cells.
Biological Information
ImmunogenSynthetic peptide corresponding to RAD50.
HostRabbit
SpecificityThis antibody recognizes RAD50.
Species Reactivity
  • Mouse
  • Human
  • Rat
Species Reactivity NoteTested on human. Predicted to react with mouse and rat based on sequence homology
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • EC 3.6.-.-
  • OTTHUMP00000065928
  • RAD50-2
  • hRAD50
  • hRad50
Purification MethodAffinity Purfied
UniProt Number
UniProt SummaryFUNCTION:Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation. Ref.5 Ref.6 Ref.7 Ref.11 Ref.13 Ref.17

Cofactor Binds 1 zinc ion per homodimer By similarity.

SUBUNIT STRUCTURE: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis. Ref.1 Ref.8 Ref.12 Ref.16 Ref.19

SUBCELLULAR LOCATION: Nucleus. Telomere. Note= Localizes to discrete nuclear foci after treatment with genotoxic agents. Ref.8 Ref.9

TISSUE SPECIFICTY: Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts. Ref.1

DOMAIN:The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc-hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer By similarity.

PTM: Phosphorylated upon DNA damage, probably by ATM or ATR By similarity.

MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.

SEQUENCE SIMILARITY:Belongs to the SMC family. RAD50 subfamily.

Contains 1 zinc-hook domain.

SEQUENCE CAUTION: The sequence AAH62603.1 differs from that shown. Reason: Miscellaneous discrepancy. Contaminating sequence. Potential poly-A sequence.
Molecular Weight~154 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceWestern Blot Analysis:
A 1:500 dilution of this antibody was used to detect RAD50 on human spleen lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8ºC from date of receipt.
To reconstitute the antibody, centrifuge the antibody at a moderate speed (5,000 rpm) for 5 minutes. Carefully remove the ammonium sulfate/PBS buffer solution and discard; 10μL of residual ammonium sulfate solution will not affect the re-suspension of the antibody. Do not let the protein pellet dry, as severe loss of antibody reactivity can occur. Re-suspend the antibody pellet in 100L either standard PBS or TBS (pH 7.3-7.5). DO NOT VORTEX. Mix by gentle stirring with a wide pipet tip or gentle finger-tapping. Let the precipitated antibody rehydrate for 1 hour at 4-25°C prior to use. Small particles of precipitated antibody that fail to re-suspend are normal. Vials are overfilled to compensate for any losses.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
07-1781 04053252677861