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05-623-25UG Anti-RNA polymerase II Antibody, clone CTD4H8

Anti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors UniProt Number: P24928
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05-623-25UG
25 μg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, R, M, Yeast (S. cerevisiae)WB, ChIP-seqMPurifiedMonoclonal Antibody
Description
Catalogue Number05-623-25UG
Brand Family Upstate
Trade Name
  • Upstate
DescriptionAnti-RNA polymerase II Antibody, clone CTD4H8
Background InformationRNA polymerase II (Pol II) is a multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.
References
Product Information
FormatPurified
Control
  • A431 nuclear extract.
PresentationPurified mouse IgG1 in buffer containing 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Quality LevelMQ100
Applications
ApplicationAnti-RNA polymerase II Antibody, clone CTD4H8 is a high quality Mouse Monoclonal Antibody for the detection of RNA polymerase II and has been published in more than 40 citations and validated for use in ChIP & WB.
Key Applications
  • Western Blotting
  • ChIP-seq
Biological Information
ImmunogenPeptide containing 10 repeats of YSPT[pS]PS corresponding to the carboxyl-terminal domain of RNA polymerase II.
CloneCTD4H8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityThis antibody recognizes Phospho- and non-phospho-RNA polymerase II.
IsotypeIgG1
Species Reactivity
  • Human
  • Rat
  • Mouse
  • Yeast (S. cerevisiae)
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Purification MethodProtein G Chromatography
UniProt Number
Molecular Weight210-220 kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by western blot on HeLa nuclear extracts.

Western Blot Analysis:
0.05-1 μg/mL of this lot detected RNA polymerase II in HeLa nuclear extracts. 0.1-2 μg/mL of a previous lot detected RNA polymerase II in 3T3 nuclear extracts
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
Packaging Information
Material Size25 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-623-25UG 04054839350191

Documentation

Anti-RNA polymerase II Antibody, clone CTD4H8 SDS

Title

Safety Data Sheet (SDS) 

Anti-RNA polymerase II Antibody, clone CTD4H8 Certificates of Analysis

TitleLot Number
Anti-RNA polymerase II, clone CTD4H8 - 3174755 3174755
Anti-RNA polymerase II, clone CTD4H8 - 3230980 3230980
Anti-RNA polymerase II, clone CTD4H8 - 3286829 3286829
Anti-RNA polymerase II, clone CTD4H8 - 3536051 3536051
Anti-RNA polymerase II, clone CTD4H8 - 3710691 3710691
Anti-RNA polymerase II, clone CTD4H8 - 3840860 3840860
Anti-RNA polymerase II, clone CTD4H8 - 4035810 4035810
Anti-RNA polymerase II, clone CTD4H8 - 4102570 4102570
Anti-RNA polymerase II, clone CTD4H8 - 4163017 4163017