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MABC679 Anti-RUNX1 Antibody, clone 2B5

Anti-RUNX1 Antibody, clone 2B5 is a highly specific mouse monoclonal antibody & that targets RUNX & has been tested in western blotting.

Anti-RUNX1 Antibody, clone 2B5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Research Sub-Category: Apoptosis - Additional Gene Symbol: RUNX1, AML1, CBFA2 UniProt Number: Q01196
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MABC679
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
HWBMAscitesMonoclonal Antibody
Description
Catalogue NumberMABC679
DescriptionAnti-RUNX1 Antibody, clone 2B5
Alternate Names
  • Runt-related transcription factor 1
  • Acute myeloid leukemia 1 protein
  • Core-binding factor subunit alpha-2
  • CBF-alpha-2
    Oncogene AML-1
  • Polyomavirus enhancer-binding protein 2 alpha B subunit
  • PEA2-alpha B
  • PEBP2-alpha B
  • SL3-3 enhancer factor 1 alpha B subunit
  • SL3/AKV core-binding factor alpha B subunit
Background InformationRunt-related transcription factor 1 (RUNX1), also known as Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2 (CBF-alpha2), Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit (PEA2-alpha B/PEBP2-alpha B), SL3-3 enhancer factor 1 alpha B subunit, and SL3/AKV core-binding factor alpha B subunit is encoded by the human gene named RUNX1/AML1, CBFA2. RUNX1 is a transcription factor that binds to a wide variety of promoters and enhancers belonging to many types of genes from T cell receptor genes to LCK, IL-3 and GM-CSF. Its major normal role is in the development of blood cells and hematopoiesis. It is expressed in most tissues except in normal brain and heart. Levels are highest in the bone marrow, thymus and peripheral lymphocytes. Defects and or translocations involving RUNX1 are the cause for myeloid leukemias as well as lymphoblastic leukemia. Mutations in RUNX1 function are also linked with many breast cancers. Finally, mutations in RUNX1 have recently been shown to be the cause of cancers in epithelial tissues and linked to squamous cell carcinomas. EMD-Millipore’s Anti-RUNX1 mouse monoclonal antibody has been tested in western blot on Jurkat cell lysates and in ELISA using recombinant RUNX1 protein.
References
Product Information
FormatAscites
Control
  • Jurkat cell lysate
PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RUNX1 Antibody, clone 2B5 is a highly specific mouse monoclonal antibody & that targets RUNX & has been tested in western blotting.
Key Applications
  • Western Blotting
Biological Information
ImmunogenSynthesized peptide of human RUNX1.
Clone2B5
HostMouse
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • RUNX1
  • AML1
  • CBFA2
Purification MethodUnpurified
UniProt Number
Molecular Weight~55 kDa observed. Uncharacterized bands may appear in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected RUNX1 in Jurkat cell lysate.

Optimal working dilutions must be determined by end user.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABC679 04053252917394