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MABT182 Sigma-AldrichAnti-Rab5A Antibody, clone 7D8.1

This Anti-Rab5A Antibody, clone 7D8.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of Rab5A.

Anti-Rab5A Antibody, clone 7D8.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Research Category: Cell Structure Research Sub-Category: Adhesion (CAMs) Gene Symbol: RAB5A, RAB5 UniProt Number: P20339

Replacement Information

Species Reactivity	Key Applications	Host	Format	Antibody Type
H	WB, IHC	M	Purified	Monoclonal Antibody

Description
Catalogue Number	MABT182
Description	Anti-Rab5A Antibody, clone 7D8.1
Alternate Names	Ras-related protein Rab-5A Rab5
Background Information	Rab5A is a membrane associated Ras related GTPase that is required for endosome fusion with the plasma membrane. Rab5A also contributes to filopodia extension and other cellular cytoplasmic extensions. Rab5A plays important roles in cellular differentiation, neurogenesis, exocytosis and protein transport. Rab proteins are a large family of GTP-binding proteins that regulate cellular membrane traffic and organelle identity. When membrane bound, each Rab has a very specific cellular location and recognizes distinct subsets of intracellular membranes, which allows Rab proteins to act as as key cellular regulators, determining the recruitment of downstream effectors to the correct membrane at the correct time. Recent research indicates that the localization of Rab proteins happens by sensing the stored curvature elastic energy in the membrane and the specific RabGEFs (Rab-activating guanosine diphosphate/guanosine triphosphate exchange factors) that associate with a particular Rab. Rab5A associates with Rabex-5 and this association provides the determinants for Rab5A specific membrane targets.

References

Product Information
Format	Purified
HS Code	3002 15 90
Presentation	Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality Level	MQ300

Applications
Application	This Anti-Rab5A Antibody, clone 7D8.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of Rab5A.
Key Applications	Western Blotting Immunohistochemistry
Application Notes	Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Rab5A in human testis tissue.

Biological Information
Immunogen	KLH-conjugated linear peptide corresponding to human Rab5A.
Clone	7D8.1
Concentration	Please refer to lot specific datasheet.
Host	Mouse
Isotype	IgG1κ
Species Reactivity	Human
Antibody Type	Monoclonal Antibody
Entrez Gene Number	NP_004153
Gene Symbol	RAB5A RAB5
Purification Method	Protein G Purified
UniProt Number	P20339
Molecular Weight	~24 kDa observed.

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements
Quality Assurance	Evaluated by Western Blotting in HEK-293 cell lysate. Western Blotting Analysis: 0.5 µg/mL of this antibody detected Rab5A in 10 µg of HEK-293 cell lysate.
Usage Statement	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage and Shipping Information
Storage Conditions	Stable for 1 year at 2-8°C from date of receipt.

Packaging Information
Material Size	100 µg

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
Catalog Number	GTIN
MABT182	04053252983658

Title	Lot Number
Anti-Rab5A, clone 7D8.1 - Q2443130	Q2443130
Anti-Rab5A, clone 7D8.1 - 3219897	3219897

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