Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

05-389-AF647 Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate is an antibody against Rac1 for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: RAC1, TC25, MIG5 UniProt Number: P63000
View Products on Sigmaaldrich.com
05-389-AF647
100 µL  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
R, H, MICCMAlexaFluor®647Monoclonal Antibody
Description
Catalogue Number05-389-AF647
DescriptionAnti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate
Alternate Names
  • Ras-related C3 botulinum toxin substrate 1
  • Cell migration-inducing gene 5 protein
  • Ras-like protein TC25
  • p21-Rac1
  • Rac1
Background InformationRas-related C3 botulinum toxin substrate 1 (UniProt P63000; also known as Cell migration-inducing gene 5 protein, p21-Rac1, Rac-1, Ras-like protein TC25, TC-25) is encoded by the RAC1 (also known as TC25) gene (ORF MIG5; Gene ID 5879) in human. Rac1, RhoA, and Cdc42 (UniProt P63000, P61586, and P60953, respectively) are among the most studied Rho family of small GTPases that are involved in a wide variety of cellular functions such as cell polarity, vesicular trafficking, and cell cycle by regulating intracellular actin dynamics. In mammals, the Rho family contains 22 members which can be further divided into the Rac subfamily (Rac1, Rac2, Rac3, and RhoG), Cdc42 subfamily (Cdc42, TC10, TCL, Chip, and Wrch-1), RhoA subfamlly (RhoA, RhoB, and RhoC) and other Rho-GTPases (RhoE/Rnd3, RhoH/TTF, Rif, RhoBTB1, RhoBTB2, Miro-1, Miro-2, RhoD, Rnd1, and Rnd2). Akt is reported to negatively regulate mixed-lineage kinase 3 (MLK3) by phosphorylating Rac1 at Ser71.
References
Product Information
FormatAlexaFluor®647
PresentationPurified mouse antibody conjugate in PBS with 15 mg/ml BSA and 0.1% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate is an antibody against Rac1 for use in Immunocytochemistry.
Key Applications
  • Immunocytochemistry
Application NotesThe unconjugated antibody (Cat. No. 05-389) is shown to be suitable also for Western blotting, immunohistochemistry, and immunoprecipitation applications.
Biological Information
ImmunogenRecombinant protein containing the full length human Rac.
Clone23A8
ConcentrationPlease refer to lot specific datasheet.
HostMouse
SpecificityRecognizes Rac1; Strong Rac1 preference with slight Rac2 cross-reactivity.
IsotypeIgG2b
Species Reactivity
  • Rat
  • Human
  • Mouse
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • RAC1
  • TC25
  • MIG5
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~21 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Rac1 in HeLa cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
05-389-AF647 04055977294910

Documentation

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate SDS

Title

Safety Data Sheet (SDS) 

Anti-Rac1 Antibody, clone 23A8, Alexa Fluor® 647 Conjugate Certificates of Analysis

TitleLot Number
Anti-Rac1, clone 23A8, Alexa Fluor® 647 Conjugate - 3173789 3173789
Anti-Rac1, clone 23A8, Alexa Fluor® 647 Conjugate - 3647668 3647668
Anti-Rac1, clone 23A8, Alexa Fluor® 647 Conjugate - 4104157 4104157
Anti-Rac1, clone 23A8, Alexa Fluor® 647 Conjugate -Q2611350 Q2611350
Anti-Rac1, clone 23A8, Alexa Fluor®647 Conjugate 2957901

Brochure

Title
New Products Flyer- Vol 4 Signaling Feature- NEW! Phosphohistidine Antibody

Posters

Title
Post-Translational Modifications