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ABN427 Anti-RalDH3 (ALDH1A3)

Anti-RalDH3 (ALDH1A3), Cat. No. ABN427, is a highly specific rabbit polyclonal antibody that targets RalDH3/ALDH1A3 and has been tested in Immunofluorescence and Western Blotting.

Anti-RalDH3 (ALDH1A3) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Aldh1a3, Aldh6, Raldh3 UniProt Number: Q9JHW9
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ABN427
100 µL  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
M, H, RIF, WBRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABN427
DescriptionAnti-RalDH3 (ALDH1A3)
Alternate Names
  • Aldehyde dehydrogenase family 1 member A3
  • Aldehyde dehydrogenase 6
  • RALDH-3
  • RalDH3
  • Retinaldehyde dehydrogenase 3
Background InformationAldehyde dehydrogenase family 1 member A3 (EC 1.2.1.5; UniProt Q9JHW9; also known as Aldehyde dehydrogenase 6, RALDH-3, RalDH3, Retinaldehyde dehydrogenase 3) is encoded by the Aldh1a3 (also known as Aldh6, Raldh3) gene (Gene ID 56847) in murine species. All-trans-retinoic acid (atRA) is a small molecule metabolite of retinol, also known as vitamine A, that plays an important role in the growth and development of many major organ systems, including the postnatal eye growth. The three retinaldehyde dehydrogenases, RALDH1-3 or ALDH1 1-3, catalyze the irreversible oxidation of retinaldehyde to form atRA. While retinaldehyde is formed via the reversible oxidation of retinol catalyzed by retinol dehydrogenases (RDHs). The three RALDH enzymes cooperatively mediate atRA signaling during eye development through tightly regulated spatiotemporal expression patterns. In the postnatal human eye, RALDH catalytic activity can be detected in the choroid, retinal pigment epithelium (RPE), and retina. RALDH2 is most abundant in the choroid and RPE, moderate abundance in the retina, and in relatively low abundance in sclera. RALDH1 is most abundant in the choroid, in moderate abundance in the sclera, and substantially reduced in the retina and RPE. RALDH3 is most abundantly expressed during embryonic eye development, but not detectable in adult ocular fundal tissues.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RalDH3 (ALDH1A3), Cat. No. ABN427, is a highly specific rabbit polyclonal antibody that targets RalDH3/ALDH1A3 and has been tested in Immunofluorescence and Western Blotting.
Key Applications
  • Immunofluorescence
  • Western Blotting
Application NotesWestern Blotting Analysis: A 1:500 dilutioin from a representative lot detected RalDH3 in 10 µg of human retina tissue lysate.

Immunofluorescence Analysis: A 1:150 dilutioin from a representative lot immunostained trabecula analge of the developing eye in paraffin-embedded embryonic E15 Sprague Dawley (SD) rat head sections by fluorescent immunohistochemistry (Courtesy of Anna Ashton, University of Aberdeen).

Western Blotting Analysis: A 1:1,500 dilutioin from a representative lot detected RalDH3 in 50 µg of new born (postnatal P0) Sprague Dawley (SD) rat whole eye tissue lysate, but not in adult SD rat brain lateral cortex and pons tissue lysates (Courtesy of Anna Ashton, University of Aberdeen).
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of mouse RalDH3.
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Mouse
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • Aldh1a3
  • Aldh6
  • Raldh3
Purification MethodAffinity purified.
UniProt Number
Molecular Weight~53 kDa observed. 55.98/56.03/56.04 kDa (human/mouse/rat) calculated (Met1 removed).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in mouse retina tissue lysate.

Western Blotting Analysis: A 1:500 dilutioin of this antibody detected RalDH3 in 10 µg of mouse retina tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABN427 04054839057793