Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

AB5585-I Anti-Recoverin Antibody

Anti-Recoverin Antibody detects level of Recoverin and has been published and validated for use in Western Blotting, Immunohistochemistry.

Anti-Recoverin Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Sensory & PNS Gene Symbol: RCVRN, RCV1 UniProt Number: P35243
View Products on Sigmaaldrich.com
AB5585-I
100 μg  
Purchase on Sigma-Aldrich

Special Offers

Overview

Replacement Information

Special Offers

100% Performance Guaranteed

Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, RWB, IHCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberAB5585-I
DescriptionAnti-Recoverin Antibody
Alternate Names
  • Cancer-associated retinopathy protein
  • Cancer-associated retinopathy antigen
  • Protein CAR
  • Recoverin
Background InformationRecoverin (UniProt P35243; also known as Cancer-associated retinopathy antigen, Cancer-associated retinopathy protein, Protein CAR) is encoded by the RCVRN (also known as RCV1) gene (Gene ID 5957) in human. The photoreceptor protein recoverin is a member of the EF-hand neuronal calcium sensor (NCS) proteins. Recoverin is initially produced as a 200-amino acid protein whose N-terminal initiator methionine is removed posttranslationally. Recoverin is composed of four EF-hand domains (a.a. 25-60, 61-96, 97-132, 147-182), with the second and third EF-hand regions mediating low- and high-affinity calcium binding, respectively. In photoreceptor cells, recoverin functions as a Ca2+-dependent inhibitor of G-protein-coupled receptor kinase 1 (GRK1). Recoverin plays an important role in mediating calcium-dependent rod and cone light adaptation. Recoverin delays the rod & cone response under dim light when [Ca2+] is high by inhibiting GRK1-catalyzed visual pigment phosphorylation, but has little effect during light adaptation when [Ca2+]i is lowered.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-Recoverin Antibody detects level of Recoverin and has been published and validated for use in Western Blotting, Immunohistochemistry.
Key Applications
  • Western Blotting
  • Immunohistochemistry
Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Recoverin in human and rat retina tissue sections.
Biological Information
ImmunogenKLH-conjugated linear peptide corresponding to a sequence from the EF-hand 4 domain of human recoverin.
EpitopeEF-hand 4
ConcentrationPlease refer to lot specific datasheet.
HostRabbit
Species Reactivity
  • Human
  • Rat
Species Reactivity NoteHuman, Rat. Predicted to react with Mouse based on 100% sequence homology.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • RCVRN
  • RCV1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~23 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in human retina tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Recoverin in 10 µg of human retina tissue lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
AB5585-I 04055977314182

Documentation

Anti-Recoverin Antibody SDS

Title

Safety Data Sheet (SDS) 

Anti-Recoverin Antibody Certificates of Analysis

TitleLot Number
Anti-Recoverin - 3208174 3208174
Anti-Recoverin - 3423493 3423493
Anti-Recoverin - 3894980 3894980
Anti-Recoverin - 3916202 3916202
Anti-Recoverin - 3957021 3957021
Anti-Recoverin - 4001594 4001594
Anti-Recoverin - 4021525 4021525
Anti-Recoverin - 4026462 4026462
Anti-Recoverin - 4037081 4037081
Anti-Recoverin - 4054784 4054784
Show more

Brochure

Title
Neuroscience Solutions for productive research