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ABS492 Anti-RhoGAP1 Antibody

Anti-RhoGAP1 Antibody is an antibody against RhoGAP1 for use in western blotting & ICC.

Anti-RhoGAP1 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Cell Cycle, DNA Replication & Repair Gene Symbol: ARHGAP1, CDC42GAP, RHOGAP1 UniProt Number: Q07960
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ABS492
100 µg  
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Key Specifications Table

Species ReactivityKey ApplicationsHostFormatAntibody Type
H, M, RWB, ICCRbAffinity PurifiedPolyclonal Antibody
Description
Catalogue NumberABS492
DescriptionAnti-RhoGAP1 Antibody
Alternate Names
  • Rho GTPase-activating protein 1
  • CDC42 GTPase-activating protein
  • GTPase-activating protein rhoOGAP
  • Rho-related small GTPase protein activator
  • Rho-type GTPase-activating protein 1
  • p50-RhoGAP
  • RhoGAP1
Background InformationRhoGAP1, also known as Rho GTPase-activating protein 1, CDC42 GTPase-activating protein, Rho-related small GTPase protein activator, Rho-type GTPase-activating protein 1, or p50-RhoGAP, and encoded by the gene ARHGAP1/CDC42GAP/RHOGAP1, is an important GTPase activator for the Rho, Rac, and CDC42 GTP binding proteins, converting them to the putatively inactive GDP-bound state. CDC42 is the preferred substrate for RhoGAP1. Such GTPase-activating proteins or GAPs play an important role in signal transduction pathways regulated by GTP-binding proteins. In addition to acting as down-regulators of GTPases, there is growing evidence that they also act as effector molecules required for downstream signaling. RhoGAP1 is found in multiprotein complexes in the cytoplasm in most cells. Recent research has shown RhoGAP1 to be particularly important during epithelial to mesenchymal morphogenesis and for carcinoma metastasis. Rho-ROCK signaling is essential for cell apical detachment and the generation of motility. RhoGAP1 restricts Rho activation to apical areas and thus helps guide the detachment process and thus is a target of much developmental and cancer research.
References
Product Information
FormatAffinity Purified
PresentationPurified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.
Quality LevelMQ100
Applications
ApplicationAnti-RhoGAP1 Antibody is an antibody against RhoGAP1 for use in western blotting & ICC.
Key Applications
  • Western Blotting
  • Immunocytochemistry
Application NotesWestern Blotting Analysis: 0.5 µg/mL from a representative lot detected RhoGAP1 in 10 µg of A431, NIH/3T3, and PC-12 cell lysates.

Immunocytochemistry Analysis: A representative lot from an independent laboratory detected RhoGAP1 in HeLa cells (Schiel, J. A., et al. (2012). Nat Cell Biol. 14(10):1068-1078.).
Biological Information
ImmunogenGST-tagged recombinant protein corresponding to human RhoGAP1.
ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
HostRabbit
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • ARHGAP1
  • CDC42GAP
  • RHOGAP1
Purification MethodAffinity Purfied
UniProt Number
Molecular Weight~50 kDa observed
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected RhoGAP1 in 10 µg of HeLa cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABS492 04053252956188