Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

MABF3085-100UG Anti-SARS-CoV-2 Spike RBD Antibody, clone 1A4-3F8

Anti-SARS-CoV-2 Spike RBD, clone 1A4-3F8, Cat. No. MABF3085, is a mouse monoclonal antibody that detects the receptor binding domain of SARS-CoV-2 spike protein and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.

View Products on Sigmaaldrich.com
MABF3085-100UG
100 μg  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberMABF3085-100UG
DescriptionAnti-SARS-CoV-2 Spike RBD Antibody, clone 1A4-3F8
Alternate Names
  • Spike glycoprotein
  • S glycoprotein
  • E2
  • Peplomer protein
Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion.
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV-2 Spike RBD, clone 1A4-3F8, Cat. No. MABF3085, is a mouse monoclonal antibody that detects the receptor binding domain of SARS-CoV-2 spike protein and is tested for use in Immunofluorescence, Immunoprecipitation, and Western Blotting.
Key Applications
  • Western Blotting
  • Immunoprecipitation
  • Immunofluorescence
Application NotesTested Applications

Immunoprecipitation Analysis: 2 µg from a representative lot immunoprecipitated SARS-CoV-2 spike glycoprotein in HEK293T S-P2A-GFP (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

Western Blotting Analysis: 0.1 µg from a representative lot detected SARS-CoV-2 spike glycoprotein in lysates from HEK293T cells expressing full-length spike protein of SARS-CoV-2 (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

Immunofluorescence Analysis: A representative lot detected SARS-CoV-2 spike glycoprotein in HEK293T cells expressing full-length spike protein of SARS-CoV-2 (Data courtesy of Dr. Stefan Schüchner, Dr. Egon Ogris, Max Perutz Labs, Medical University of Vienna, Austria).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment from the C-terminal region of the receptor binding domain (RBD) of SARS-CoV-2 Spike protein.
EpitopeC-terminal
Clone1A4-3F8
Concentration1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 1A4-3F8 is a mouse monoclonal antibody that specifically detects receptor binding domain (RBD) of SARS-CoV-2 Spike protein. It targets an epitope within the C-terminal region of RBD.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteSARS-CoV-2
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • S
Purification MethodProtein G purified
UniProt Number
Molecular Weight~180 kDa observed; 141.18 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in lysate from HEK293T cells expressing full-length SARS-CoV-2 spike protein.

Western Blotting Analysis (WB): A 1:250 dilution of this antibody detected SARS-CoV-2 spike glycoprotein in lysate from HEK293T cells expressing full-length SARS-CoV-2 spike protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF3085-100UG 04065268529027