Millipore Sigma Vibrant Logo
Attention: We have moved. EMD Millipore products are no longer available for purchase on emdmillipore.com.Learn More

ABF1066-100UL Anti-SARS-CoV-2 spike protein ectodomain (S1+S2)

Anti-SARS-CoV-2 spike protein ectodomain (S1+S2), Cat. No. ABF1066, is a rabbit polyclonal antibody that detects the extracellular domain (S1+ S2) of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralizing, and Western Blotting.

View Products on Sigmaaldrich.com
ABF1066-100UL
100 µL  
Purchase on Sigma-Aldrich

Overview

Replacement Information
Description
Catalogue NumberABF1066-100UL
DescriptionAnti-SARS-CoV-2 spike protein ectodomain (S1+S2)
Alternate Names
  • Spike glycoprotein
  • S glycoprotein
  • E2
  • Peplomer protein
Background InformationSpike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. This polyclonal antibody binds and neutralizes the SARS-CoV-2 variants including the B.1.1.7; B.1.429; P.1; and B.1.351 at different levels. (Ref.: Ravichandran, S., et al. (2020). Sci Transl Med. 12(550):eabc3539).
References
Product Information
FormatSerum
PresentationRabbit polyclonal antibody in serum without preservatives.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV-2 spike protein ectodomain (S1+S2), Cat. No. ABF1066, is a rabbit polyclonal antibody that detects the extracellular domain (S1+ S2) of SARS-CoV-2 spike protein and is tested for use in ELISA, Neutralizing, and Western Blotting.
Key Applications
  • Western Blotting
  • Neutralizing
  • Enzyme Immunoassay (ELISA)
Application NotesTested Applications

Enzyme Immunoassay (ELISA) Analysis: A representative lot detected SARS-CoV-2 spike protein ectodomain (S1+S2) in ELISA applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Neutralizing: A representative lot neutralized SARS-CoV-2 spike protein ectodomain (S1+S2) in Neutralizing applications (Ravichandran, S., et. al. (2020). Sci Transl Med. 12(550):eabc3539).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Biological Information
ImmunogenHis-tagged recombinant fragment corresponding to the extracellular domain (S1 + S2; aa 16-1213) of SARS-CoV-2 spike protein.
EpitopeExtracellular domain
ConcentrationPlease refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostRabbit
SpecificityThis rabbit polyclonal antibody detects the extracellular domain (S1+ S2) of SARS-CoV-2 spike protein.
Species Reactivity
  • Virus
Species Reactivity NoteSARS-CoV-2 virus.
Antibody TypePolyclonal Antibody
Entrez Gene Number
Gene Symbol
  • S
Purification MethodUnpurified
UniProt Number
Molecular Weight~170 kDa observed; 139.13 kDa calculated for spike protein. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant extracellular domain (S1+S2) of SARS-CoV-2 spike protein.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected recombinant extracellular domain (S1 + S2) of Spike glycoprotein of SARS-CoV-2.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Packaging Information
Material Size100 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
ABF1066-100UL 04065266059984