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MABF2212-100UG Anti-SARS-CoV Nsp8 Antibody, clone 5A10

Anti-SARS-CoV Nsp8, clone 5A10, Cat. No. MABF2212, is a mouse monoclonal antibody that detects Nsp8 of SARS-C0V and is tested for use in Immunofluorescence and Western Blotting.

NewMABF2212-100UG
100 μg  
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Overview

Replacement Information
Description
Catalogue NumberMABF2212-100UG
DescriptionAnti-SARS-CoV Nsp8 Antibody, clone 5A10
Alternate Names
  • Nonstructural protein NS8
  • ORF8
Background InformationNonstructural protein NS8 (UniProt: A0A6B9VTP3; also known as ORF8 protein) is encoded by the orf8 (also known as NS8) gene (Gene ID: 43740577) in Sars-CoV-2. Coronaviruses (CoV) represent a diverse family of positive-sense RNA viruses capable of causing respiratory and enteric disease in human and animal hosts. Amongst these the SARS-CoV-2 is the most prominent virus that causes severe respiratory syndrome in human. The nonstructural proteins (nsps) of coronavirus assemble to form the replication transcription complex (RTC) responsible for viral RNA synthesis. In SARS-CoV-2 non-structural protein Nsp8 is a critical component of the RNA replicase and features both well folded secondary structure and disordered segments. It is part of the RNA-dependent RNA polymerase (RdRp) complex and forms a heterotetramer consisting of one molecule of Nsp7, two copies of Nsp8 and one of Nsp12. Nsp8 an Nsp7 are reported to adopt a hollow cylinder-like structure and serve as co-factors for the catalytic Nsp12 that play a role in the stabilization of Nsp12 regions involved in RNA binding and are essential for a highly active Nsp12 polymerase complex. (Ref.: Trevino, MA., et al. (2023). Nucleic Acids Res. 51(18); 10041-10048; Couroble, VV., et al. (2021). J. Am. Soc. Mass Spectrom. 32(7); 1618-1630; Kumar, P., et al. (2007). Virology. 366; 293-303).
References
Product Information
FormatPurified
PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Quality LevelMQ200
Applications
ApplicationAnti-SARS-CoV Nsp8, clone 5A10, Cat. No. MABF2212, is a mouse monoclonal antibody that detects Nsp8 of SARS-C0V and is tested for use in Immunofluorescence and Western Blotting.
Key Applications
  • Western Blotting
  • Immunofluorescence
Application NotesWestern Blotting Analysis: A representative lot detected Nsp8 in Western Blotting applications (Kumar, P., et al. (2007). Virology. 366(2):293-303).

Immunofluorescence Analysis: A representative lot detected NSP8 in Immunofluorescence applications (Kumar, P., et al. (2007). Virology. 366(2):293-303).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Biological Information
ImmunogenGST-tagged recombinant Nonstructural protein NS8 from SARS-CoV-2.
EpitopeUnknown
Clone5A10
Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
HostMouse
SpecificityClone 5A10 is a mouse monoclonal antibody that detects Nonstructural protein NS8 of SARS-CoV-2.
IsotypeIgG1κ
Species Reactivity
  • Virus
Species Reactivity NoteVirus (SARS-CoV)
Antibody TypeMonoclonal Antibody
Entrez Gene Number
Gene Symbol
  • orf8
Purification MethodProtein G purified
UniProt Number
Molecular Weight~22 kDa observed; 13.83 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting with recombinant SARS-CoV-2 Nsp8 protein.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected recombinant SARS-CoV-2 Nsp8 protein.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage: +2°C to +8°C.
Packaging Information
Material Size100 μg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalog Number GTIN
MABF2212-100UG 04065270845221